利拉鲁肽对2型糖尿病大鼠NF-κB及VCAM-1表达的影响  

Effect of liraglutide on the expressions of NF-κB and VCAM-1 in aorta endothelium of type 2 diabetic rats

作　　者：綦才辉[1] 金勇君[1] 杨美子[2] 徐鑫淼[1] 张凌云[1] 曹鹏娟 

机构地区：[1]滨州医学院烟台附属医院内分泌科,山东烟台264100 [2]滨州医学院药理学教研室,山东烟台264003

出　　处：《山东大学学报（医学版）》2014年第4期39-42,48,共5页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(30660070);山东省优秀中青年科学家科研奖励基金(BS2010YY002)

摘　　要：目的观察利拉鲁肽对2型糖尿病大鼠主动脉核因子-κB(NF-κB)及血管细胞间黏附因子(VCAM-1)表达的影响及可能机制。方法高脂高糖饲料喂养联合小剂量链脲佐菌素腹腔注射,建立2型糖尿病大鼠模型。40只8周龄雄性SD大鼠分为4组:正常对照组(NC组)、糖尿病对照组(DM组)、格列本脲对照组(DMG组)及利拉鲁肽干预治疗组(DML组)。DMG组及DML组分别给予格列本脲及利拉鲁肽干预治疗8周。12周末腹主动脉取血分离血清,测空腹血糖(FBG)、空腹胰岛素(FIns)、甘油三酯(TG)、胆固醇(TC)、低密度脂蛋白(LDL-C)及高密度脂蛋白(HDL-C);分离大鼠主动脉,石蜡切片HE染色观察大鼠主动脉形态学变化;免疫组化方法(SP法)测大鼠主动脉NF-κB及VCAM-1的表达。结果与DM组相比,DMG组及DML组大鼠FIns及胰岛素抵抗指数(HOMA-IR)明显降低(P均<0.01);与DMG组相比,DML组大鼠FIns、HOMA-IR、TG及LDL-C明显降低(P均<0.05),主动脉NF-κB和VCAM-1的表达降低(P<0.05)。结论利拉鲁肽可下调糖尿病大鼠主动脉NF-κB和VCAM-1的表达,调节糖脂代谢。此过程可改善大血管并发症。Objective To investigate the effect of liraglutide on the expressions of nuclear factor-kappa B （ NF-κB） and vascular cell adhesion molecule 1 （VCAM-1） in aorta endothelium of type 2 diabetic rats and the possible mechanism. Methods Forty male SD rats were divided into the normal control group （NC group）, diabetic control group （DM group）, glibenclamide group （DMG group） and liraglutide group （DML group）.The model of type2 diabetic rats was eatablised by injecting with low-dose streptozocin（ STZ） into abdominal cavity of SD rats after high-fat and high-sugar diet.Then rats in DMG and DML groups were treated with liraglutide and glibenclamide for 8 weeks.At the end of the twelfth week, blood was taken from abdominal aorta to examine biochemical indexes including fasting blood-glucose （FBG）, fasting insulin（FIns）, totalcholesterol（TC）, triglyceride（TG）, low density lipoprotein-cholesterol（LDL-C） and high density lipoprotein-cholesterol （ HDL-C） .The thoracic aorta was isolated to observe the histological parame-ters by HE staining.The expressions of NF-κB and VCAM-1 in aorta endothelium were determined by immunohisto-chemical method.Rusults The levels of FIns and HOMA-IR in DMG group and DML group were decreased com-pared with those in DM group （P＆lt;0.01）.The levels of FIns, HOMA-IR,TG and LDL-C and the expressions of NF-κB and VCAM-1 in in aorta endothelium in DML group were decreased compared with those in DMG group （ P＆lt;0.05）.Conclusion Liraglutide can decrease the expressions of NF-κB and VCAM-1 in aorta endothelium of type2 di-abetic rats and improve glucolipid metabolism.This may be involved in vascular complications of diabetes.

关 键 词：利拉鲁肽 糖尿病大鼠 血管细胞间黏附因子1 VASCULAR cell adhesion MOLECULE 1 

分 类 号：R587.1[医药卫生—内分泌]