高剂量法诱导胃癌铂类耐药细胞株的建立及其生物学特性  

Establishment of cisplatin-resistant gastric cancer cell line and the biological characteristics

作　　者：延冰[1] 刘国勤[2] 姜永胜[2] 沈海玉[2] 孙少川[2] 

机构地区：[1]山东大学医学院,山东济南250012 [2]山东大学附属济南市中心医院胃肠外科,山东济南250013

出　　处：《山东大学学报（医学版）》2014年第4期49-52,57,共5页Journal of Shandong University:Health Sciences

基　　金：济南市科技计划项目(201101114)

摘　　要：目的探讨利用高剂量(0.8μg/mL)顺铂(cDDP)诱导胃癌顺铂耐药细胞株的建立及其生物学特性。方法采用大剂量冲击法诱导人胃癌耐顺铂细胞株BGC823/cDDP建立。观察BGC823/cDDP细胞形态,CCK-8法检测细胞的耐药指数,CCK-8绘制生长曲线,流式细胞仪检测细胞的生长周期,Transwell法检测细胞侵袭能力的变化,免疫发光法测定细胞培养液中肿瘤标志物CEA、CA-199含量。结果采用大剂量cDDP冲击方法诱导后,BGC823/cDDP细胞较BGC823细胞体积略增大,细胞核增大,形状不规则,并可见散在的多核巨细胞,其对顺铂的耐药指数为4.54,耐药细胞系的倍增时间较正常BGC823细胞延长,BGC823/cDDP细胞G1期细胞增多,而S、G2期细胞减少,耐药细胞株侵袭力增强(P<0.05),耐药细胞培养液中肿瘤标志物CEA、CA-199含量与正常胃癌细胞无明显差别。结论通过大剂量cDDP冲击方法建立了顺铂耐药胃癌细胞株BGC823/cDDP,耐药细胞与正常细胞的生物学特性不同。Objective To explore the drug-resistant gastric cancer cell line induced by high-dose cDDP and its biologi-cal characteristics.Methods A drug-resistant gastric cancer cell line BGC823/cDDP was established by high-dose cD-DP.The changes of biological characteristics of the cell lines were observed using light microscopy.The resistance in-dex was determined by CCK-8 assay.The cell growth curve was drawn by CCK-8 assay.The cell cycle of drug-resist-ant cells was tested by flow cytometry.The cell invasion ability was observed by Transwell assay.The densities of CEA and CA-199 in the cell culture medium were evaluated by immune luminescence.Results Compared with BGC823 cells, the BGC823/cDDP cells turned to be slightly bigger and irregular in shape, and some giant cells could be seen. The proliferation rate of BGC823/cDDP cell prolonged, with the resistance index being 4.54.The population doubling-time prolonged.The cell numbers of G1 phase increased while decreased in S and G2 phase in BGC823/cDDP cell.The invasion ability was stronger in BGC823/cDDP cell （P＆lt;0.05）.The densities of CEA and CA-199 had no significant differences between BGC823 and BGC823/cDDP.Conclusion The cDDP-resistant gastric cancer cell line BGC823/cDDP was induced by high-dose cDDP.The BGC-823 and BGC-823/cDDP cells were different in biological character-istics.

关 键 词：胃癌 耐药细胞 顺铂 生物学特性 

分 类 号：R735.2[医药卫生—肿瘤]