结直肠癌组织中miRNA的差异表达研究  

Differential expression of microRNAs in human colorectal cancer tissues

作　　者：严秀蕊[1] 王立斌[1] 李玉奎[1] 杨银学[1] 

机构地区：[1]宁夏医科大学总医院宁夏人类干细胞研究所,银川750004

出　　处：《重庆医科大学学报》2014年第3期295-298,共4页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(编号:81260309);宁夏自然科学基金资助项目(编号:NZ13152);宁夏医科大学校级资助项目(编号:XZ2011001)

摘　　要：目的:寻找并鉴定在结直肠癌及癌旁正常组织中差异表达的miRNA,为进一步阐明其在结直肠癌发生、发展机制中的作用奠定实验基础。方法:收集10例新鲜的结直肠癌及癌旁正常组织,抽提总RNA,利用miRNA芯片技术寻找结直肠癌及癌旁正常组织中差异表达的miRNA,并通过real-time PCR技术进行验证。结果:与结直肠癌旁正常组织相比,结直肠癌组织中上调2.3倍以上的miRNA有32个,下调2倍以上的miRNA有10个;real-time PCR结果与miRNA芯片结果较吻合。结论:筛选得到结直肠癌miRNA差异表达谱,其可能与结直肠癌的发生、发展相关。Objective：To identify the differentially expressed microRNAs （miRNAs） in human colorectal cancer tissues and to make further study on the function of miRNA in pathogenesis and development mechanism of colorectal cancer. Methods：Total RNA was extracted from 10 samples fresh colorectal cancer tissues and normal adjacent tissues and the differentially expressed miRNAs were detected by miRNA array. Real-time PCR was applied to verify the reliability of miRNA array results. Results：miRNA array results showed that 32 miRNAs were up-regulated more than 2.3 folds and 10 miRNAs were down-regulated more than 2 folds in coloreetal cancer tissues compared with those in normal adjacent colorectal tissues. And the real-time PCR results were in accordance with the miRNA array results. Conclusion ： The miRNA differential expression profile of colorectal cancer is obtained, which might relate with the pathogenesis and development of colorectal cancer.

关 键 词：MIRNA芯片 结直肠癌 MICRORNAS 

分 类 号：R735.3[医药卫生—肿瘤]