ERRα mRNA及蛋白在乳腺癌中的表达及其临床意义  

mRNA and protein expression of estrogen receptor-related receptor α in breast cancer and its clinical significance

作　　者：李鹍鹏[1] 刘胜春[1] 

机构地区：[1]重庆医科大学附属第一医院内分泌乳腺外科,重庆400016

出　　处：《重庆医科大学学报》2014年第3期324-327,共4页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(编号:30871295)

摘　　要：目的:研究雌激素受体相关受体α(estrogen receptor-related receptorα,ERRα)mRNA和蛋白在乳腺癌组织及乳腺癌旁组织的表达及意义,进一步探讨ERRα与乳腺癌临床病理指标[雌激素受体(estrogen receptor,ER)、孕激素受体(progesterone receptor,PR)、人表皮生长因子受体-2(human epidemic growth factor receptor-2,Her-2)、P53],以及与乳腺癌分级、分期的相关性。方法:收集乳腺癌及对应乳腺癌旁组织标本87例,分别采用免疫组织化学染色,RT-PCR,Western blot检测ERRαmRNA和蛋白的表达情况,进一步分析ERRα的表达与乳腺癌临床病理特征的关系。结果:本研究中ERRαmRNA和蛋白在乳腺癌组织中的表达均高于在乳腺癌旁组织中的表达(P<0.05),且ERRα的表达与乳腺癌病理指标Her-2、ER以及肿瘤的病理分级,肿瘤大小,淋巴结转移相关,而与患者的年龄、PR、P53等无明显相关。结论:ERRα在乳腺癌中的高表达,可能作为乳腺癌一个预后不良的新的分子标记物,为乳腺癌的诊治提供新的思路。Objective：To detect the expression and significance of estrogen receptor-related receptor（ERR） α mRNA and protein in breast cancer and breast para-carcinoma tissues and to further discuss the correlation between ERR α and breast cancer clinical pathological index（ （estrogen receptor（ER）,progestin receptor（PR）,human epidermal growth factor receptor-2（Her-2）,P53） as well as the grade and classification of breast cancer. Methods：Expression of ERRα in 87 breast cancer tissues and the corresponding breast para-carcinoma tissues were detected by immunohistochemistry and reverse transcription PCR（RT-PCR） ,Western blot respectively and their relationship with the clinical pathological characteristics was analyzed. Results ： Expression of ERR α mRNA and protein in breast cancer tissues was higher than that in breast para-carcinoma tissues（P〈0.05）. Expression of ERR α was correlated with ER, Her-2, degrees of differentiation, tumor size and metastasis of the lymph nodes but was not correlated with patient＇ s age, PR and P53. Conclusions ： High expression of ERR α may serve as a biomarker of poor prognosis in breast cancer and it may provide new thought for the diagnosis and treatment of breast cancer.

关 键 词：ERRα 乳腺癌 免疫组织化学 RT-PCR WESTERN BLOT 

分 类 号：R737.9[医药卫生—肿瘤]