石榴皮鞣花酸对4T1乳腺癌小鼠免疫功能的影响  

Influence of pomegranate peel ellagic acid on immune function of mice with 4T1 breast cancer

作　　者：张玉梅[1] 王家晓[1] 

机构地区：[1]广西中医药大学第一附属医院,广西南宁530023

出　　处：《现代中西医结合杂志》2014年第15期1597-1599,1602,共4页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：广西自然科学基金项目(2010GXNSFA013213);广西青年基金项目(2011GXNSFB018104);广西中医药大学院级重点课题(2D2009003);广西中医药大学院级课题(P2009129)

摘　　要：目的探讨石榴皮鞣花酸对人乳腺癌4T1细胞株生长的抑制作用及对乳腺癌荷瘤小鼠免疫功能的影响。方法测定石榴皮鞣花酸对体外培养的4T1细胞生长的抑制率;流式细胞仪测定肿瘤细胞凋亡情况;检测应用石榴皮鞣花酸后荷瘤小鼠的脾淋巴细胞的NK、LAK、CTL活性及血清TNF活性改变,并与对照组比较。结果石榴皮鞣花酸抑制4T1细胞生长形成;流式细胞仪显示石榴皮鞣花酸可有效诱导细胞凋亡;石榴皮鞣花酸低剂量组和高剂量组均可明显诱导荷瘤小鼠脾淋巴细胞NK、LAK和CTL活性提高,且血清TNF水平明显上升,与生理盐水组、DDP组比较均有显著性差异(P均<0.05)。石榴皮鞣花酸低剂量组与高剂量组均无显著性差异(P>0.05)。结论石榴皮鞣花酸可明显抑制4T1细胞增殖诱导其凋亡,石榴皮鞣花酸可明显诱导提高荷瘤小鼠特异性抗肿瘤免疫反应。Objective It is to observe the inhibitory action of pomegranate peel ellagic acid on human 4T1 breast cancer cell and its influence on immune function in breast tumor-bearing mice. Methods The growth inhibition rate of pomegranate peel ellagic acid on 4T1 cell cultured in vitro was detected;the tumor cell apoptotic ratio was measured by flow cytometry;the changes of NK,LAK,CTL activity in spleen lymphocytes and serum TNF of tumor-bearing mice were detected after treated by pomegranate peel ellagic acid,and compared with the control group. Results The growth and formation of 4T1 cells were in-hibited by pomegranate peel ellagic acid. Flow cytometry showed that pomegranate peel ellagic acid could induce apoptosis ef-fectively. Pomegranate peel ellagic acid low-dose and high-dose groups could significantly enhance the induction of tumor-bear-ing mouse spleen lymphocytes NK,LAK and CTL activity,and the serum TNF levels were significantly increased,there were significant difference compared with the saline group or DDP group（P〈0. 05）. There was no significant difference between pomegranate peel ellagic acid low-dose and high-dose groups（P〉0. 05）. Conclusion Pomegranate peel ellagic acid can in-hibit the proliferation and induce apoptosis in 4T1 cells. Pomegranate peel ellagic acid can significantly improve tumor-bearing mice induce specific anti-tumor immune response.

关 键 词：石榴皮鞣花酸 乳腺癌 小鼠 免疫功能 

分 类 号：R737.9[医药卫生—肿瘤]