姜黄素通过Hedgehog信号通路抑制胶质瘤细胞增殖  

Curcumine inhibits the proliferation of glioma cells through Hedgehog signaling pathway

作　　者：窦志金[1] 杜文众[1] 刘幸[1] 孙颖[1] 谢彩玲 明键光[1] 陈辉[1] 蒋传路[1] 

机构地区：[1]哈尔滨医科大学附属第二医院神经外科,150086 [2]漯河市第二人民医院药剂科,462000

出　　处：《中国微侵袭神经外科杂志》2014年第5期228-231,共4页Chinese Journal of Minimally Invasive Neurosurgery

基　　金：黑龙江省自然基金资助(编号:ZJY0604-02);卫生部基金项目:(编号:w2011BX059)

摘　　要：目的探讨姜黄素通过Hedgehog信号通路对胶质瘤细胞增殖的影响。方法采用细胞毒性检测方法检测姜黄素对不同种胶质瘤细胞的细胞毒性作用;通过实时定量PCR、Western-blotting分析和免疫荧光检测姜黄素对Hedgehog信号通路中重要成分(Shh、Smo、GLI1)表达的影响;采用MTT实验、细胞集落形成实验观察姜黄素对胶质瘤增殖能力的影响。结果随姜黄素浓度增加,姜黄素对胶质瘤细胞系的细胞毒性作用增强。姜黄素作用于胶质瘤细胞后,Hedgehog信号(Shh、Smo、GLI1)mRNA和蛋白表达水平下降。姜黄素抑制GLI1转移进入细胞核同时减少其受体集聚。姜黄素抑制胶质瘤细胞增殖及细胞集落形成,并显示出浓度和时间依赖性。结论姜黄素通过下调Hedgehog信号通路中的重要成分(Shh、Smo、GLI1)抑制胶质瘤细胞增殖。Objective To explore the effect of curcumine on glioma proliferation by the Hedgehog signaling pathway. Methods Cytotoxicity assay was conducted to detect the cytotoxicity of curcumine on the different kinds of glioma cells. The influence of curcumine on the important components （Shh, Smo, GLI1） in Hedgehog signaling pathway was investigated by real-time quantitative PCR, Western-blotting and immunofluorescence assay. MTT assay and colony formation assay were performed to determine the effect of curcumine on cell proliferation at different doses and times. Results In glioma cell line, with the increase of the concentration of curcumine, the cytotoxic effect of curcumine was enhanced. Both mRNA and protein levels of Hedgehog signaling components （Shh, Smo, GLI1） were down-regulated based on the effect of curcumine on glioma cells. Curcumine suppressed GLI1 translocation into cell nucleus and reduced the assembly of its receptors. Glioma cell proliferation and colony forming were inhibited by curcumine, which displayed the concentration- and time-dependence. Conclusions Curcumine inhibits glioma cell proliferation through the down- regulation of the important components （Shh, Smo, GLI 1） in Hedgehog signaling pathway.

关 键 词：神经胶质瘤 HEDGEHOG信号通路 姜黄素 增殖 

分 类 号：R739.4[医药卫生—肿瘤]