高效液相色谱法测定虫草中核苷类和麦角甾醇  

Determination of nucleosides and ergosterol incaterpillar fungus by HPLC

作　　者：周苏[1,2] 邢增涛[2] 饶钦雄[2] 

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海市农业科学院农产品质量标准与检测技术研究所,上海201403

出　　处：《上海农业学报》2014年第2期60-64,共5页Acta Agriculturae Shanghai

基　　金：上海科委技术标准专项(10DZ0503400)资助

摘　　要：运用高效液相色谱建立了同时测定冬虫夏草及北虫草中5种核苷类物质的检测方法,该方法对目标物回收率较高,线性良好,操作简单易行。对北虫草及3个不同产地冬虫夏草测定结果表明:北虫草含有5种核苷类物质,而3个产地冬虫夏草中均未检出虫草素,北虫草中虫草素含量为(1.89±0.06)mg/g;4种虫草样品均含麦角甾醇,其中北虫草含量最多为(4.58±0.07)mg/g,青海冬虫夏草含量最少为(1.61±0.07)mg/g。A method to simultaneously determine 5 kinds of nucleosides of Cordyceps sinensis and natural caterpillar fungus was established by high performance liquid chromatography （HPLC）, not only having a high recovery rate and good linearity but also being simple and easy in operation. The determination of 5 nucleosides and ergosterol contents of Cordyceps sinensis and natural caterpillar fungi from 3 different habitats showed that Cordyceps sinensis contained 5 nucleosides （inosine, guanosine,adenine, adenosine, and cordycepin）and its cordycepin content was （1.89 ± 0.06） mg/g while all the 3 natural caterpillar fungi had no cordycepin being detected;All the 4 caterpillar fungus samples contained ergosterol,the greatest content was （4.58 ±0.07） mg/g of Cordyceps sinensis and the least content was （l. 61 ± 0.07） mg/g of natural caterpillar fungus from Qinghai Province.

关 键 词：虫草 核苷 麦角甾醇 高效液相色谱法 

分 类 号：S567.3[农业科学—中草药栽培]