新型铈苦味酸配合物的合成、表征及其与白蛋白相互作用  

Synthesis,Characterization and Interaction of a Novel Ce( Ⅲ) Picorate Complex with HSA,BSA and VOA

作　　者：霍丽娜[1] 刘伟[1] 杨天林[1] 李晓红[1] 

机构地区：[1]宁夏大学能源化工重点实验室化学化工学院,宁夏银川750021

出　　处：《稀土》2014年第2期37-43,共7页Chinese Rare Earths

基　　金：国家自然科学基金项目(21261018);教育部科学基金重点项目(208195)

摘　　要：合成了一种新的配体2,7-二(二苯胺酰乙氧基)萘及其铈苦味酸盐的配合物,采用1HNMR、红外光谱、元素分析、摩尔电导及热分析对其结构进行了表征,推测配合物的组成为[Ce(pic)3L2]。在pH=7.30的TrisHCl缓冲溶液中采用荧光光谱法分别研究了配合物与HSA、BSA、VOA的作用方式。结果表明,配合物对3种白蛋白的荧光猝灭属于静态猝灭,且猝灭程度为HSA>BSA>VOA。计算了不同温度下配合物与3种白蛋白间的结合常数(KA)、结合位点(n≈1)及相关热力学参数(ΔH<0,ΔS>0,ΔG<0),确定配合物与3种白蛋白之间主要靠静电作用力结合。并且采用同步荧光光谱及三维荧光光谱法研究了配合物对HSA、BSA和VOA构象的影响。A new ligand 2,7- Bis（ N- phenylaniline carbonyl ethoxy） naphthaedi and its picorate complexe [Ce（ pic）3] L2were synthesized and characterized by 1HNMR,IR spectra,elemental analysis and molar conductivity. The interaction of picorate Ce（ Ⅲ） complexe with HSA,BSA and VOA has been studied by fluorescence spectrum in Tris- Hcl buffer solution（ pH = 7. 30）. The results indicated that the Ce（ Ⅲ） complex caused the fluorescence quenching of HSA,BSA and VOA through a static quenching procedure and that the quenching degree was HSA〉 BSA〉 VOA. The binding constants（ KA）, the number of binding sites（ n≈1）,and corresponding thermodynamic parameters enthalpy change（ ΔH〈 0）,entropy change（ ΔS〉 0）,Gibbs free energy change（ ΔG 〈0）,between Ce（ Ⅲ） complexe and HAS（ BSA /VOA） at different temperatures were calculated. The primary binding pattern between Ce（ Ⅲ） complex and HSA（ BSA / VOA） were interpreted as electrostatic interaction. The effect of Ce（ Ⅲ） complexe conformation of HAS,BSA and VOA were investigated by synchronous fluorescence spectra and three- dimensional fluorescence spectra.

关 键 词：稀土配合物 荧光猝灭 白蛋白 

分 类 号：O614.332[理学—无机化学]