氟离子对莱茵衣藻和蛋白核小球藻的生理效应  

Physiological effect of fluoride ions on Chlamydomonas reinhardtii and Chlorella pyrenoidosa

作　　者：李潜[1] 吴沿友[2,3] 吴运东[3] 

机构地区：[1]江苏大学环境与安全工程学院,镇江212013 [2]中国科学院地球化学研究所环境地球化学国家重点实验室,贵阳550002 [3]江苏大学农业工程研究院,镇江212013

出　　处：《环境化学》2014年第5期776-781,共6页Environmental Chemistry

基　　金：国家自然科学基金项目(40973060)资助

摘　　要：以莱茵衣藻(Chlamydomonas reinhardtii)和蛋白核小球藻(Chlorella pyrenoidosa)为受试生物,研究了F-对藻叶绿素含量、胞外碳酸酐酶(CAex)活性及稳定碳同位素组成(δ13C)的影响,以探讨F-对微藻的生理效应.结果表明,当F-浓度不超过10 mmol·L-1时,随F-浓度增大,蛋白核小球藻叶绿素a、b含量和CAex活性基本不变,而莱茵衣藻叶绿素a、b含量降低,CAex活性小幅增加;当F-浓度在10—200 mmol·L-1之间时,随F-浓度的增加,蛋白核小球藻和莱茵衣藻的叶绿素a、b含量均明显下降,CAex活性显著增大,且在F-浓度为100 mmol·L-1时达最大,200 mmol·L-1时两种藻均不能生存,无CAex活性和叶绿素含量表征.莱茵衣藻对F-具有更灵敏的CAex响应机制,对F-的影响更为敏感,而蛋白核小球藻对F-的耐受性更好;在实验浓度范围内,莱茵衣藻和蛋白核小球藻的δ13C值均随F-浓度的增大呈现出更大的负偏差,F-促使二者优先利用较轻的12C,说明两种微藻光合作用固定无机碳模式发生改变.Using Chlamydomonas reinhardtii and Chlorella pyrenoidosa as tested organisms, effects of fluoride ion （ F-） on chlorophyll （ Chl） content, extracellular carbonic anhydrase （ CAex ） activity and stable carbon isotope composition were investigated, to understand the physiological effects of F-on microalgae. Results showed that when F-concentration was below 10 mmol·L-1 , Chl-a and Chl-b contents of C. reinhardtii decreased, while CAex activity of the algae increased with increasing F-concentration. In contrast, those of C. pyrenoidosa were almost unchanged. When F- concentration was between 10 and 200 mmol·L-1 , Chl-a and Chl-b contents of the two algae decreased significantly, whereas CAex activity of them increased with the F- concentration and reached maximum at 100 mmol·L-1 F- concentration. When F- concentration was 200mmol·L-1 , the two algae were not able to survive, and chlorophyll content and CAex activity could not be detceted. C.reinhardtii was more sensitive to F-, Whereas C. pyrenoidosa had a higher tolerance to F-. In the range of the concentrations studied, the values of δ13 C of the two algae were all more negatively skewed than those of the controls, indicating that the algal cells preferred the utilization of the lighter 12 C isotope in the presence of F-. Thus ways of the photosynthetic utilization of inorganic carbon in＆amp;nbsp;the two algae have changed.

关 键 词：F- 莱茵衣藻 蛋白核小球藻 叶绿素 胞外碳酸酐酶 δ13C. 

分 类 号：X173[环境科学与工程—环境科学]