应用组织工程方法修复缺损面肌的动物实验研究  

The Application of Tissue Engineering Methods to Repair the Defect of Musculus Facialis:an Animal Experiment Research

作　　者：刘坤[1] 王薇[1] 王少新[1] 李超[1] 唐休发[2] 何等旗[3] 华成舸[2] 张兵[1] 

机构地区：[1]四川省肿瘤医院头颈外科,四川成都610041 [2]四川大学华西口腔医学院,四川成都610041 [3]兰州大学第一医院口腔科,甘肃兰州730000

出　　处：《四川医学》2014年第5期528-530,共3页Sichuan Medical Journal

基　　金：四川省科技厅应用基础课题(编号:2006J13-131)

摘　　要：目的本研究拟通过用组织工程方法修复缺损面肌,为头颈外科肿瘤术后遗留缺损的动态修复提供新的理论和方法。方法应用细胞培养技术,组织工程方法将SD大鼠成肌细胞与I型胶原复合、培养,并将组织工程化的材料植入成年大鼠面部与面神经断端接触,形成运动单位,以期开发出能替代缺损面肌的仿真生物材料。结果本研究为骨骼肌组织工程中的血管化和神经化提供了重要的参考,其血管化方法可用于其他临床缺血性疾病治疗的研究。故本研究的结果不仅对骨骼肌工程神经支配的问题作出了初步的回答,进一步将在更广的范围里,对血管化和血管生成的研究产生重要影响。结论用组织工程方法修复缺损面肌将会为头颈外科肿瘤术后遗留缺损的修复提供新的选择。Objective To investigate tissue engineering methods which repair facial defect,it provides a new method for postoperative patients of head and neck surgery.Methods Through cell culture technique and tissue engineering,cultivate myoblast of SD rat with type I collagen,then the tissue-engineered materials be implanted to facial nerve ending of adult rat,to form the motor unit,in order to develop biological materials to repair the defect in the facial muscles.Results This study has made a preliminary answer of the innervation of skeletal muscle engineering problems,it also influences the study of vascularization and angiogenesis.Conclusion Using tissue engineering methods to repair facial defect of postoperative patients of head and neck surgery will provides a new choice for us.

关 键 词：组织工程 种子细胞 成肌细胞 

分 类 号：R739.91[医药卫生—肿瘤]