骨髓间充质干细胞移植联合电针治疗对脑出血大鼠脑组织中BMP2表达的影响  

Effect of combined treatment of mesenchymal stem cells transplantation and electric acupuncture on the expression of BMP2 in rats with intracerebral hemorrhage

作　　者：杨国强[1] 萧洪文[1] 范学慧[2] 范光碧[1] 李振[1] 王玉波[1] 杨朝鲜[3] 

机构地区：[1]泸州医学院解剖学教研室,四川泸州646000 [2]泸州医学院心血管医学研究所,四川泸州646000 [3]泸州医学院神经生物学研究室,四川泸州646000

出　　处：《泸州医学院学报》2014年第2期164-168,共5页Journal of Luzhou Medical College

基　　金：四川省应用基础研究计划项目(No.2013JY0075);四川省教育厅重点项目(No.12ZA077)

摘　　要：目的:探讨骨髓间充质干细胞(MSCs)移植和电针(Ea)联合治疗大鼠脑出血性损伤对脑组织中骨形态发生蛋白-2(BMP2)表达的影响。方法:100只SD大鼠尾壳核处注射肝素和胶原酶建立脑出血动物模型,并随机分成生理盐水组(Ns组)、电针组(Ea)、MSCs组、MSCs移植联合电针组(MSCs+Ea)共4组,每组25只。术后第3 d分别给予注射生理盐水、电针刺激、MSCs移植、MSCs移植联合电针刺激,每组又根据治疗时间的不同分为1、3、5、7、14 d五个亚组,每个亚组5只。到相应时间点时麻醉处死大鼠,取脑制作冰冻切片,采用免疫组化方法检测BMP2的表达。结果:对各组BMP2阳性细胞数比较显示:MSCs+Ea组各时间点比Ns组相应时间点增多(P<0.05),在1、5、7 d时比Ea组上升(P<0.05),在1 d和14 d时比MSCs组增加(P<0.05);MSCs组比Ns组在3 d和5 d时增多,比Ea组在1 d和5 d时上调(P<0.05)。Ns组和Ea组相比,P>0.05,差异无统计学意义。结论:MSCs移植和电针联合治疗比单纯的MSCs移植或电针刺激更能促进脑出血大鼠脑组织中BMP2的表达。Objective： To explore the effect of combined treatment of mesenchymal stem cells（MSCs）transplantation and electric acupuncture（Ea） on the expression of BMP2 in rats with intracerebral hemorrhage（ICH）. Methods： 100 rat ICH models were set up by injecting collagenase and heparin into the caudate putamen nucleus in rats. The rats were randomly divided into four groups（n = 25）： Ns group, Ea group, MSCs group and MSCs ＋ Ea group. Each group was divided into 1 d,3 d,5 d,7 d and 14 d subgroups. The rat brains were took out and frozen sections were processed. The expression of BMP2 was detected by immunohistochemistry. Results：BMP2 expression of MSCs ＋ Ea group was higher than that of Ns group at all subgroups（P〈 0.05）, higher than that of Ea group at 1 d, 5 d and 7 d（P 〈 0.05） and higher than MSCs group at 1d and 14 d（P 〈 0.05）. The BMP2of MSCs group raised remarkably compared with Ns group at 3 d and 5 d（P 〈 0.05）, and more remarkable than that of Ea group at 1 d and 5 d（P 〈 0.05）. BMP2 cells had no significant difference between Ns group and Ea group（P〉0.05）. Conclusion： The combined treatment of MSCs transplantion and electric acupuncture promotes the expression of BMP2 in ICH rats.

关 键 词：骨髓间充质干细胞 电针 BMP2 脑出血 

分 类 号：R741.05[医药卫生—神经病学与精神病学] R363[医药卫生—临床医学]