PSCAmRNA在前列腺上皮内瘤的表达与前列腺癌发生发展密切相关  

Expression of prostate stem cell antigen mRNA in prostatic intraepithelial neoplasia:predictor of the development of prostate cancer

作　　者：赵志刚[1] 何军[1] 张镜伟 曾国华[1] 沈文律[2] 

机构地区：[1]广州医科大学附属第一医院泌尿外科,510230 [2]汕头大学医学院第二附属医院泌尿外科

出　　处：《现代泌尿生殖肿瘤杂志》2014年第2期107-111,115,共6页Journal of Contemporary Urologic and Reproductive Oncology

基　　金：国家自然科学基金项目(81072112;81372774);广州市属高校羊城学者科研项目(12A014G)

摘　　要：目的研究前列腺干细胞抗原(PSCA)mRNA表达与前列腺上皮内瘤(PIN)发生恶性变的关系。方法收集前列腺活检诊断为孤立性PIN患者117例,其中低级别PIN(LGPIN)51例,高级别PIN(HGPIN)66例。通过核酸原位杂交技术检测PIN及其继发前列腺癌的PSCA mRNA表达。卡方检验和t检验分析PSCA mRNA在组间的表达差异。单变量和多变量Logistic回归分析评价PSCA mRNA表达对PIN进展为前列腺癌的风险。结果根据Gleason评分,在34例继发前列腺癌中,PSCA mRNA表达水平高于相应的PIN且差异有统计学意义(P<0.001)。与LGPIN相比,HGPIN的PSCA mRNA表达水平的增高明显且差异有统计学意义(P<0.01)。相比没有发展为前列腺癌的LGPIN和HGPIN,发展为前列腺癌者的PSCA mRNA的表达水平明显增高(P<0.001和P<0.001)。多变量的Logistic回归分析证明仅PSCA mRNA可以分别预测由孤立性LGPIN及孤立性HGPIN进展为前列腺癌。结论 PIN中PSCA mRNA的表达可以作为其进展为临床前列腺癌的一个重要预测指标,提示PSCA在前列腺肿瘤发生过程中有重要意义。Objective To determine whether prostate stem cell antigen （PSCA）mRNA ex-pression was associated with the presence of cancer in prostatic intraepithelial neoplasia （PIN）. Methods One hundred seventeen men were diagnosed with isolated PIN on initial prostate biopsy, 51 with low-grade form （LGPIN）,and 66 with high-grade form （HGPIN）.PSCA mRNA expres-sion in initial PIN and subsequent cancer was examined by in situ hybridization.The differences of the PSCA mRNA expression level between the groups were analyzed by the Chi-square and Student＇s t-test.Univariate and multivariate logistic regression analyses were performed to evaluate the pre-dictive performance of PSCA mRNA. Results PSCA mRNA expression level in 34 subsequent cancers was statistically increased compared with their paired PIN （P〈0.001）,with a Gleason＇s de-pendence.HGPIN showed statistically high PSCA mRNA expression compared with LGPIN （P〈0.01）.PSCA mRNA expression levels were significantly stronger in the initial isolated LGPIN and i-solated HGPIN with subsequent cancer than those without （P〈0.001 and P〈0.001,respectively）. Multivariate logistic regression analysis demonstrated that only PSCA mRNA was predictive of the onset of subsequent cancer in patients with isolated LGPIN and in those with isolated HGPIN,re-spectively. Conclusions Our data identify PSCA mRNA in initial PIN as a significant predictor of subsequent cancer,suggesting that PSCA play a importance role in prostatic tumorigenesis.

关 键 词：前列腺肿瘤 前列腺干细胞抗原 前列腺上皮内瘤 

分 类 号：R737.25[医药卫生—肿瘤]