原生质体紫外诱变选育达托霉素高产菌株  

Screening of High Daptomycin-Producing Strain by Protoplast UV Mutation

作　　者：段向东[1] 贾啸静[1] 陈丽华[1] 仲伟潭[1] 段宝玲[1] 

机构地区：[1]华北制药集团新药研究开发有限责任公司微生物药物国家工程研究中心 河北省微生物代谢工程技术研究中心,河北石家庄050015

出　　处：《化学与生物工程》2014年第5期59-62,共4页Chemistry & Bioengineering

摘　　要：研究了达托霉素产生菌玫瑰孢链霉菌D-30原生质体制备及再生的条件。结果显示,D-30原生质体制备及再生的最适条件为:在种子培养基中添加0.6%的甘氨酸,当种子培养30h后,用浓度为2mg·mL-1的溶菌酶破壁,酶解温度为32℃,酶解时间为75min。在最适条件下制得的原生质体经紫外诱变处理后培养,最终筛选得到高产菌株D-35,该菌株的平均发酵单位较出发菌株提高了87.9%。In order to select a high daptomycin-producing strain,the method of protoplast treatment by UV mutation was established.Factors affecting protoplast preparation,regeneration and UV mutation were studied. The optimum preparation and regeneration conditions of protoplast were as follows：Streptomyces roseosporus D-30was cultured for 30hin the medium containing 0.6% of glycine,then the mycelia were processed with 2 mg·mL-1 of lysozyme at 32℃for 75min.The as-prepared protoplasts of Streptomyces roseosporus were cultured after UV mutation,and a high daptomycin-producing mutant strain D-35was obtained.Compared with the original strain,the production of daptomycin increased by 87.9%.This study provides an useful method to screen high daptomycin-producing strain.

关 键 词：达托霉素 玫瑰孢链霉菌 原生质体 紫外诱变 

分 类 号：TQ927[轻工技术与工程—发酵工程]