复配酶解法提取鸭硫酸软骨素工艺研究  

Extraction Technology of Compound Enzyme Hydrolysis of Chondroitin Sulfate from Duck

作　　者：陈晨[1] 王宝维[1,2] 葛文华[2] 张名爱[1] 岳斌[2] 韩海娜[1] 

机构地区：[1]青岛农业大学食品科学与工程学院,山东青岛266109 [2]青岛农业大学优质水禽研究所,山东青岛266109

出　　处：《中国家禽》2014年第9期29-34,共6页China Poultry

基　　金：国家水禽产业技术体系专项基金(CARS-43-11);山东省农业重大应用技术创新课题(2013)

摘　　要：为了探索硫酸软骨素的新型提取资源和更加环保的提取技术,试验以42日龄肉鸭胸软骨为研究对象,研究优化了复配酶解法提取鸭硫酸软骨素的工艺。试验采用单因素和响应面法,优化了鸭胸软骨除杂处理和酶法提取鸭硫酸软骨素的工艺。结果表明:采用甲醇、氯仿(V/V=2∶1)和1 mol/L NaCl溶液处理鸭胸软骨表面的油脂和杂蛋白效果最好;鸭胸软骨酶解液中杂蛋白的最佳沉降剂为9%TCA溶液;复合蛋白酶和胰蛋白酶复配提取硫酸软骨素效果最佳,配比为1∶0.5;鸭胸软骨粉复合酶解的最佳条件为:酶解温度48.53℃,酶解时间5.90 h,酶解pH 6.45。在该条件下,鸭硫酸软骨素的提取率达到46.02%,纯度达到96.39%。To explore new resources and environmental technology,the optimum compound emzymolysis technology was studied for chondroitin sulfate extracted from breast cartilages of 42-day-age ducks. The optimum conditions of deimpurity treatment and enzymatic method of breast cartilage were analyzed by single factor experiments and response surface optimization. The results showed that：Being immersed by methanol and chloroform with the ratio of 2∶1,then stirring with 1 mol/L NaCl was found to be the optimum condition for removing surface grease and redundant proteins from breast cartilage;9% TCA solution was the superior sinking agent for removing redundant proteins of enzymatic hydrolysate for breast cartilages;The optimum enzyme for hydrolyzing was the mixture of protease and trypsin with the ratio of 1∶0.5;The optimum compound enzymolysis conditions were as follows：hydrolysis temperature of 48.53 ℃,hydrolyzing time of 5.90 h,pH 6.45. The extraction ratio was 46.02% and purity level was 96.39% under this optimumcondition.

关 键 词：鸭胸软骨 化学法 生物酶法 硫酸软骨素 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]