地衣芽孢杆菌WX-02补硝酸钠发酵产聚γ-谷氨酸工艺  

Optimizing Sodium Nitrate Fed-batch Culture on Poly(γ-glutamic acid) Fermentation by Bacillus licheniformis WX-02

作　　者：许尚华 胡丽芳[1] 陈守文[1] 

机构地区：[1]华中农业大学农业微生物国家重点实验室,武汉430070

出　　处：《湖北农业科学》2014年第4期903-906,共4页Hubei Agricultural Sciences

摘　　要：以地衣芽孢杆菌(Bacillus licheniformis)WX-02为研究对象,以提高其发酵产生的聚γ-谷氨酸的产量为研究目的,经摇瓶发酵得到优化的硝酸钠补料方案,即初始硝酸钠浓度为10 g/L,发酵至12 h时补加5 g/L硝酸钠时聚γ-谷氨酸产量最大。将此补料方案在3 L发酵罐上进行验证。结果表明,发酵得到的菌体生物量和聚γ-谷氨酸产量最大值分别为4.5 g/L和38.7 g/L,与对照相比分别提高55.2%和14.8%。通过补硝酸钠可以提高菌体生物量和对谷氨酸的利用率,从而提高聚γ-谷氨酸的发酵水平。Bacillus licheniformis WX-02 was used to study the ways of improving poly（γ-glutamic acid）（γ-PGA） yield. The optimal conditions of sodium nitrate feeding for γ-PGA fermentation by Bacillus licheniformis WX-02 by flask-shaking fedbatch fermentation were the initial sodium nitrate concentration 10 g / L, and 5 g / L of sodium nitrate added when fermentation to 12 h. The fermentation was carried out in a 3 L fermentor, showing that the maximum of biomass and γ-PGA yield was 4.5 g / L and 38.7 g / L with increase of 55.2% and 14.8%, compared with the control. It is indicated that adding sodium nitrate can increase biomass, enhance the utilization of glutamate, and improve the γ-PGA fermentation.

关 键 词：地衣芽孢杆菌(Bacillus licheniformis) 聚Γ-谷氨酸 硝酸钠 补料发酵 生物量 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]