hMSC对高转移潜能肝癌模型影响的实验研究  

Effect of bone marrow-derived mesenchymal stem cells on high- metastatic potential hepatocellular carcinoma in mouse models: an intervention study

作　　者：周军[1] 李天然[1] 宋斌[1] 杜湘珂[2] 魏正茂[2] 霍天龙[2] 

机构地区：[1]南京军区福州总医院九五临床部机关,莆田351100 [2]北京大学人民医院放射科

出　　处：《功能与分子医学影像学（电子版）》2013年第2期24-28,共5页Functional and Molecular Medical Imaging(Electronic Edition)

基　　金：国家自然科学基金(81271607);福建省自然基金项目资助(2013J01392)

摘　　要：目的观察人骨髓间充质干细胞(hMSC)移植入动物模型对肝癌组织的影响情况及对肝癌转移潜能影响。方法制作高转移潜能动物模型,实验组在接种肿瘤后第7日开始尾静脉注射hMSC,5×105cells/次,2次/周,对照组尾静脉注射hMSC细胞培养液,0.2 ml/次,实验开始后每4天用测量肿瘤体积,肿瘤接种后14天(2周)、21天(3周)、28天(4周)、35天(5周)、42天(6周)处死动物,称重,取瘤块,称瘤重,计算肿瘤重量抑制率。PCR检测动物模型标本转移相关因子OPN、BSP、α-Ⅴ基因的表达,及肿瘤标本凋亡相关因子Bcl2、Bax、Caspase3基因的表达。结果在第3周时肿瘤的重量抑制率效果最好,随着时间的延长,肿瘤的抑制率逐渐下降。肝癌转移相关的生物学指标均呈逐渐下降趋势,代表肿瘤凋亡指标的因子呈两极分化表现,抗凋亡Bcl2因子呈逐渐下降的趋势,凋亡因子Bax、Caspase3呈逐渐升高的趋势。结论 hMSC对肝癌动物模型肿瘤抑制效能随时间而变化,hMSC移植后第3周对肝癌抑制效果最显著,随着时间的延长,抑制效能减弱。hMSC对肝癌的转移潜能具有抑制作用。Objective To observe therapeutic effect of hMSC transplantation into HCC animal models, and hMSC effect on metastatic potential of HCC. Methods HMSC via tail vein were injected into the nude mice in vivo. In the experimental group, hMSC （5 ×105cells） were injected by tail vein on the 7th day after tumor inoculation, 2 times a week. hMSC cell culture medium 0.2 ml/per mouse was injected by tail vein in control group. After the start of the experiment, caliper measures the subcutaneous tumor volume every 4 days. After tumor inoculation 14 days （two weeks）, 21 days （three weeks）, 28 days （4 weeks） 35 days （5 weeks）, and 42 days （6 weeks）, animal models were sacrificed and tumor weight and body weight were recorded, and then the inhibition rate were calculated. PCR detected OPN, BSP, α- V gene expression and apoptosis-related factors of Bcl2, Bax, Caspase3 gene expression of high metastatic HCC animal model tumor specimens. Results The inhibition rate of tumor weight results showed that the best tumor inhibition rate effect was in third week, and the tumor inhibition rate was gradually decreased with time delay. Metastasis-related biological factors showed a gradual down-regulated trend. But the tumor apoptotic factors were polarization, and anti-apoptotic factor Bcl2showed a decreasing trend, and apoptotic factors Bax, Caspase3 were gradually increased trend. Conclusion HMSC on the high metastatic potential of hepatocellular carcinoma animal models effectiveness changes with time. After hMSC transplantation, the inhibition performance on HCC was best in the third week, and weakened with the time delay. hMSC was significantly inhibited the metastatic potential of hepatocellular carcinoma.

关 键 词：骨髓间充质干细胞 骨桥蛋白 唾液酸蛋白 高转移潜能细胞 低转移潜能细胞 动物模型 

分 类 号：R735.7[医药卫生—肿瘤] R329.2[医药卫生—临床医学]