OLC1蛋白在非小细胞肺癌患者外周血血浆中的水平及其临床意义  

OLC1 protein levels in plasma of patients with non-small cell lung cancer and its clinical application

作　　者：杨龙海[1] 肖汀[2] 谭金晶[2] 陈实平[3] 高燕宁[2] 程书钧[2] 刘向阳[1] 孙克林[1] 

机构地区：[1]北京协和医学院中国医学科学院肿瘤医院胸外科,100021 [2]北京协和医学院中国医学科学院分子肿瘤学国家重点实验室 [3]中国医学科学院北京协和医学院基础医学研究所

出　　处：《中华肿瘤杂志》2014年第5期362-365,共4页Chinese Journal of Oncology

基　　金：国家“863”高技术研究发展计划（2012AA020206）

摘　　要：目的探讨肺癌相关蛋白OLC1在非小细胞肺癌（NSCLC）患者外周血血浆中的水平及其临床意义。方法取5～6周龄BalB／c小鼠，OLC1全长蛋白作为抗原免疫制备OLC1抗体。每只小鼠免疫4次，每间隔2周免疫1次，每次15～30峙抗原蛋白。通过Westernblot方法筛选抗体，制备检测血浆中OLC1蛋白的双抗体夹心法酶联免疫吸附试验（ELISA）试剂盒，并检测281例NSCLC患者和92例健康对照者（性别年龄匹配）血浆中OLC1蛋白的水平。采用受试者工作特征曲线（ROC曲线）下面积评估OLC1对NSCLC的检测效能。结果共制备了11株特异性好的OLC1单克隆抗体，并成功建立了检测血浆OLC1的ELISA试剂盒，检测范围为1．95～62．50ng／ml。NSCLC患者外周血血浆中位OLC1蛋白水平（124．69ng／m1）显著高于健康对照者（67．07ng／ml，P〈0．001）。OLC1区分NSCLC和健康对照者的ROC曲线下面积为0．69（95％C1为0．62～0．76），当OLC1的cutoff值为67．72ng／ml时，其灵敏度为84．4％，特异度为51．1％。OLC1区分早期（IA期）NSCLC和健康对照者的ROC曲线下面积为0．68（95％C1为0．6～0．77），其灵敏度为77．8％，特异度为54．4％。结论NSCLC患者血浆中OLC1蛋白水平显著升高，可成为辅助临床诊断的候选标志物。Objective The aim of this study was to detect the plasma concentration of OLC1 （overexpressed in lung cancer 1 ） protein as a potential cancer biomarker, and evaluating its clinical application value in the diagnosis of non-small cell lung cancer （NSCLC）. Methods We prepared OLC1 antibody with OLC1 full length protein, in 5-6-week old Bal B/c mice. Each mouse was immunized four times at a dose of 15-30 μg antigen protein, and the interval between two consecutive immunizations was two weeks. Antibody screening was made by ELISA and Western blot, and a double antibody sandwich ELISA kit was developed. We used this established ELISA kit to detect the plasma concentration of OLC1 protein in 281 NSCLC patients and 92 gender- and age-matched healthy controls. Area under the receiver operating characteristic curve （AUC） was used to evaluate the detection efficacy of OLC1. Results We obtained 11 OLC1 monoclonal antibodies and successfully established the ELISA kit to detect the plasma concentration of OLC1 with a detection range from 1.95 ng/ml to 62.50 ng/ml. OLC1 concentration in the case group （124.69 ng/ml） was significantly higher than that in the control group （67.07 ng/ml, P 〈0. 001 ）. In the scenario of distinguishing NSCLC from control group, AUC result was 0.69. When the cut-off was set at 67.72 ng,/ml, the sensitivity and specificity was 84.4% and 51.1% , respectively. In term of distinguishing early lung cancer （[A） from normal controls, the AUC, sensitivity and specificity were 0.68, 77. 8% and 54. 4%, respectively. Conclusion The plasma concentration of OLC1 protein is significantly elevated in NSCLC patients. OLC1 may be as a potential cancer biomarker applied in clinical diagnosis.

关 键 词：癌 非小细胞肺 OLC1 血浆 抗体 单克隆 早期诊断 

分 类 号：R734.2[医药卫生—肿瘤]