精神分裂症患者血浆miRNA表达水平与精神症状的关系研究  

Correlation between the microRNA expression level in plasma and psychiatric symptoms in schizophrenia patients

作　　者：宋红涛[1] 过伟[2] 孙欣羊[3] 张梁[1] 赵林[4] 钟爱芳[2] 牛威[2] 戴运华 张理义[2] 卢正斌 

机构地区：[1]江苏大学临床医学院,江苏镇江213013 [2]常州解放军102医院,江苏213003 [3]第二军医大学心理与精神病学系,上海200433 [4]徐州医学院研究生院,徐州221000 [5]GoPath Laboratories LLC, Illinois 60089, USA

出　　处：《解放军医学杂志》2014年第5期388-391,共4页Medical Journal of Chinese People's Liberation Army

基　　金：南京军区2012年医学科技创新课题(12MA002)~~

摘　　要：目的探讨精神分裂症患者血浆中miRNA表达水平与精神症状的关系。方法连续纳入2012年7月-2013年5月收治的53例未经药物治疗的精神分裂症患者,采用实时荧光定量PCR技术检测血浆中9种miRNA(miR-30e、miR-34a、miR-181b、miR-195、miR-346、miR-432、miR-7、miR-132、miR-212)的表达水平,采用阳性和阴性症状量表(PANSS)评估其精神症状,并对结果进行统计学分析。结果 miR-34a与激活性症状及攻击性症状呈显著负相关(r=–0.345、–0.284,P<0.05),miR-346与一般精神病理症状呈明显负相关(r=–0.282,P<0.05)。miR-34a高表达组激活性症状群分和攻击性症状群分显著低于低表达组(P<0.05),miR-346高表达组反应缺乏症状群分显著低于低表达组(P<0.05)。miR-34a分别进入以激活性症状群和攻击性症状群为因变量的回归方程,可解释激活性症状变异的10.2%,攻击性症状群变异的6.3%;miR-346进入以一般精神病理症状为因变量的回归方程,可解释一般精神病理变异的6.1%。结论精神分裂症患者血浆中的miR-34a与激活性症状、攻击性症状有明显关联,miR-346与一般精神病理症状有明显关联,此二者可能在上述精神症状的发生发展过程中起重要作用。Objective To explore the correlation between the microRNA expression in plasma and psychiatric symptoms in schizophrenia patients. Methods A total of 53 consecutive schizophrenia patients who had not received antipsychotic treatment from July 2012 to May 2013 were enrolled in the present study. The expressions of 9 schizophrenia-associated microRNAs （miR30e, miR-34a, miR-181b, miR-195, miR-346, miR-432, miR-7, miR-132 and miR-212） in plasma were determined by real-time fluorescence quantitative PCR, with the psychiatric symptoms evaluated by Positive and Negative Symptoms Scale （PANSS）, and the results were analyzed statistically. Results The expression level of miRNA-34a was significantly negatively correlated with the active symptoms and aggressive symptoms （r=–0.345, r=–0.284, P〈0.05）, and the expression level of miRNA-346 was significantly negatively correlated with the general psychopathologic symptoms （r=–0.282, P〈0.05）. The scores of activity and aggressiveness in the higher expression subgroup of miRNA-34a were significantly lower than those in lower expression subgroup （P〈0.05）. The score of disturbance of thought in the higher expression subgroup of miRNA-346 was significantly lower than that in lower expression subgroup （P〈0.05）. When miRNA-34a was entered into the two regression equations with the activity and aggressiveness as the independent variables respectively, it explained 10.2% of the variance of activity and 6.3% of the variance of aggressiveness. When miRNA-346 was entered into the regression equation with the general psychopathology as the independent variable, and it explained 6.1% of the variance of general psychopathology. Conclusions The expression level of miRNA-34a is highly correlated with the active symptoms and aggressive symptoms, and the expression level of miRNA-346 is highly correlated with the general psychopathological symptoms. Both miRNA-34a and miRNA-346 might play important roles in the development and progression of psychiatric sympt

关 键 词：精神分裂症 微RNAS 精神症状 

分 类 号：R749.3[医药卫生—神经病学与精神病学]