牙槽裂两侧正畸牙移动后RANKL和OPG表达的研究  

Expression of receptor activator of NF-κB ligand and osteoprotegerin in alveolar cleft after teeth moving towards defect area

作　　者：赖文佳[1] 张灵超 刘楚峰[2] 曹阳[1] 

机构地区：[1]中山大学光华口腔医学院·附属口腔医院,广东省口腔医学重点实验室,广东广州510055 [2]广东省口腔医院·南方医科大学附属口腔医院正畸科,广东广州510280

出　　处：《临床口腔医学杂志》2014年第5期262-265,共4页Journal of Clinical Stomatology

基　　金：国家自然科学基金项目(81170990;81200813);广东省科技计划项目(2012B061700107)

摘　　要：目的：研究牙移动后牙槽裂隙两侧破骨细胞核因子κB受体活化因子配体（RANKL）和骨保护素（OPG）的表达和作用.方法：选用4只4月龄雄性Beagle犬建立牙槽裂模型,正畸牵引裂隙两侧牙齿向裂隙区移动,实时荧光定量聚合酶链反应（real-time PCR）检测裂隙两侧牙槽骨中RANKL和OPG mRNA的表达水平.结果：正畸牵引16～20周后,牙槽裂间隙缩小以至关闭,原裂隙处有新骨生成.裂隙两侧牙槽骨中RANKL和OPG mRNA表达增加.结论：牙槽裂两侧牙齿受正畸牵引后,RANKL与OPG参与骨重建,裂隙处骨改建活跃.此方法为修复牙槽裂提供新的思路.Objective：To study the expression and role of receptor activator of NF-κB ligand （RANKL） and osteoprotegerin （OPG） in alveolar cleft when teeth moving towards defect area.Method：An animal model of alveolar cleft was established in 4 male Beagle dogs of 4 months old.With/Under orthodontic force,teeth beside the defect moved towards each other.Real-time PCR was used to detect the expression of RANKL and OPG mRNA in alveolar bone adjacent to the defect.Result：After 16～20 w orthodontic treatment,bone defect was narrowed and even closed,and bone formation was observed in this area.The expression of RANKL and OPG mRNA in alveolar bone adjacent to the defect was increased.Conclusion：RANKL and OPG play an important role in bone remodeling during orthodontic tooth movement to repair alveolar bone cleft.This study provide a new method of alveolar cleft treatment.

关 键 词：牙槽裂 正畸牙移动 RANKL OPG 

分 类 号：R783.5[医药卫生—口腔医学] Q786[医药卫生—临床医学]