MMP-1/PAR-1通路在宫颈癌侵袭转移中的作用  

Role of MMP-1/PAR-1 Signaling in the Cervical Cancer Invasion

作　　者：赵倩[1] 孙宁霞[1] 叶宸[1] 马妍[1] 李文[1] 

机构地区：[1]第二军医大学长征医院妇产科,上海200003

出　　处：《生殖与避孕》2014年第5期368-373,共6页Reproduction and Contraception

基　　金：上海市卫生局课题资助项目;项目号:2011160

摘　　要：目的:探索基质金属蛋白酶-1(MMP-1)/蛋白酶活化受体-1(PAR-1)通路在宫颈癌侵袭转移中的作用。方法:RT-PCR法检测59例临床宫颈鳞癌组织和15例正常宫颈组织的MMP-1mRNA表达;Transwell实验检测添加重组人MMP-1及干扰PAR-1对宫颈癌Hela细胞转移侵袭能力的影响。结果:宫颈癌组织中的MMP-1(rhMMP-1)表达高于正常宫颈组织,并与侵袭转移程度相关;rhMMP-1能促进Hela细胞的转移能力,并与浓度相关;干扰PAR-1后能抑制MMP-1介导的Hela细胞转移能力。结论:MMP-1/PAR-1通路参与宫颈癌的侵袭转移,可能成为宫颈癌治疗的新靶点。Objective： To investigate the role of matrix metalloproteinase-1 （MMP-1）/protease-activated receptors-1 （PAR-I） signaling in the cervical cancer invasion. Methods： RT-PCR was used to test the mRNA level of MMP-1 in 59 cases of cervical squamous cell cancer and 15 case of narmal cervical tissues. Transwell test was designed to explore the effect of adding recombinant human MMP-1 （rhMMP-1） and PAR-1-siRNA on cervical cancer （Hela cells） invasion. Results： In cervical cancer tissues more MMP-1 expression was observed than in normal cervical tissues, and its expression correlated with tumor status, rhMMP-1 could promote Hela cell invasion, and its number of invasive cell correlated with the concentration of rhMMP-1. Disrupting the expres- sion of PAR-1 reduced the MMP-1 promoting-effect on Hela cell invasion, but no effect on non-MMP-1 invasive action. Conclusion： The MMP-1/PAR-1 signaling is involved in cervical cancer invasion. Therefore, blocking PAR-1 may represent a new therapeutic option for metastatic cervical cancer.

关 键 词：宫颈癌 基质金属蛋白酶-1(MMP-1) 蛋白酶活化受体-1(PAR-1) 侵袭 转移 

分 类 号：R737.33[医药卫生—肿瘤]