HIF1A基因多态性与原发性肝癌预后相关性研究  

Polymorphisms in HIF1A gene are associated with prognosis of hepatocelluar carcinoma

作　　者：王侃[1] 王沈[1] 于晓河[2] 石志勇[2,3] 曲萍[4] 黄小军[4] 杨业发[2] 张洪新[1] 

机构地区：[1]第四军医大学唐都医院疼痛科,陕西西安710038 [2]第二军医大学东方肝胆外科医院放射介入一科 [3]苏州大学医学部 [4]第四军医大学教学实验中心

出　　处：《实用放射学杂志》2014年第5期830-833,共4页Journal of Practical Radiology

基　　金：国家自然科学基金项目（81272522,81071896,81270505）;国际科技合作项目（S2011GR0239）.

摘　　要：目的：研究缺氧诱导因子1α基因（hypoxia-inducible factor-1αgene,HIF1A）多态性与中晚期原发性肝癌预后的相关性。方法收集448例中晚期原发性肝癌行动脉化疗栓塞治疗患者的预后信息,并收集患者外周血5 mL,提取基因组 DNA,检测HIF1A 上3个位点（rs2301113、rs2057482和 rs1957757）的单核苷酸多态性（single nucleotide polymorphism,SNP）,多因素的COX 回归,生存曲线和 Log-rank 检验用于分析 SNPs 与肝癌预后的关系。结果在肿瘤最大径〈5 cm 的患者中,携带 rs2301113位点突变型（WV＋VV）患者的预后显著优于野生型（WW）患者（HR,0.58；95%CI,0.35－0.96；P =0.036）；在单发肿瘤患者中,携带 rs2301113突变型（WV＋VV）患者的生存率优于野生型（WW）患者（Log-rank P =0.048）。结论 HIF1A 基因多态性可作为中晚期原发性肝癌行 TACE 治疗的独立预后指标。Objective To assess the association between polymorphisms in HIF1α gene and prognosis of advanced hepatocelluar carcinoma.Methods We collected prognosis data from a cohort of 448 advanced HCC patients treated by transarterial chemoembo-lisation,and used 5ml peripheral blood from patients for extraction DNA.Three SNPs （rs2301 1 13、rs2057482 and rs1 957757 ）in HIF1αgene were selected and genotyped.Multivariate Cox proportional hazards model,Kaplan-Meier curve and log-rank test were used for prognosis analyses.Results The variant-containing genotypes （WV＋VV）of SNP rs2301 1 13 exhibited a significant associ-ation with a better overall survival in HCC patients who had tumor size smaller than 5 cm （hazard ratio [HR],0.58,95% confidence interval [CI],0.35-0.96,P =0.036）.In the patients taken single tumor subgroup,the variant-containing genotypes （WV＋VV） of SNP rs2301 1 13 exhibited a significant association with a better overall survival （log-rank P =0.048）,comparing to those carrying wild-type genotype.Conclusion Our results suggest that polymorphisms in HIF1αgene may serve as an independent prognosis bio-marker for advanced HCC patient.

关 键 词：中晚期原发性肝癌 动脉化疗栓塞 缺氧诱导因子 1α 单核苷酸多态性 

分 类 号：R735.7[医药卫生—肿瘤] R815[医药卫生—临床医学]