大鼠实验性肝癌组织中Treg细胞与TGF-β1、IL-10水平的相关性分析  

Correlation analysis of Treg cells and TGF-β1, IL-10 levels in a rat experimental liver carcinoma model

作　　者：张志强[1] 贺杰峰[2] 张瑜[1] 兰志伟[1] 赵浩亮[2] 

机构地区：[1]山西医科大学,太原030001 [2]山西医科大学附属山西大医院普外科

出　　处：《中华普通外科杂志》2014年第5期382-384,共3页Chinese Journal of General Surgery

基　　金：国家自然科学基金资助项目（30640025）

摘　　要：目的探讨大鼠肝癌Treg细胞变化与TGF-β1、IL-10的表达水平之间的相关性。方法40只雄性Wistar大鼠分成3组，肝癌模型组（20只）、盐水对照组（10只）、空白对照组（10只），肝癌模型组大鼠按100mg／ko给予腹腔注射二乙基亚硝胺（DEN）诱导肝癌模型。光镜下观察肝组织病理改变，应用流式细胞术检测肝组织Treg细胞的百分率，免疫组化SABC法检测肝组织TGF-β1和IL-10的表达水平，并对Treg细胞、TGF-β1和IL-10的变化进行相关性分析。结果制备大鼠诱导性肝癌模型，流式细胞术检测显示肝癌模型组肝癌组织中Treg细胞占CIM＋T细胞的比例为14．32％±4．84％，显著高于空白对照组的5．64％±6．10％和盐水对照组的7．95％±3．55％，差异有统计学意义（F=211．279，P〈0．05）。肝癌组织中TGF-β1和IL-10的表达高于空白对照组和盐水对照组（FTGF-β1=250．740，FIL-10=152．744，均P〈0．05）。Treg细胞与TGF-β1、IL-10的水平呈明显正相关（P〈0．05）。结论大鼠实验性肝癌组织中TGF-β1、IL-10和Treg细胞水平显著升高，Treg细胞与TGF-β1及IL-10水平显著正相关，共同参与机体对肝癌的免疫抑制作用。Objective To study the relationship between Treg cell numbers and level ofTGF-β1, IL-10 in the immune microenvironment of rat liver cancer. Method 40 male Wistar rats were randomly divided into three groups, liver cancer model group （n = 20 ） , saline control group （n = 10 ） and blank control group （n = 10 ）. Liver cancer model was established by intraperitoneal injection of DEN （ 100 mg/kg）. Percentage of Treg cells in cancer tissues was detected by fiow cytometry and the expression level of TGF-β1 and IL-10 by immunohistochemical SABC. Results The ratio of Treg ceils in CD4 ± T ceils was 14. 32% ± 4. 84% in liver cancer tissues, significantly higher than that in the blank control group 5.64% ±6. 10% and the saline control group 7.95% ± 3.55%. The difference was statistically significant （F = 211. 279, P 〈 0. 05 ）. The expression level of TTGF-β1 and IL-10 in cancer tissues was significantly higher than the blank control group and the saline control group （ FTGF-β1= 250. 740, F11-10 = 152. 744, all P 〈0. 05）. The number of Treg ceils was positively correlated with TGF-β1, IL-10 level（P 〈 0. 05）. Conclusions TGF-β1, IL-10 and Treg ceils significantly increased in rat experimental liver cancer tissues, and there was positive correlation between Treg ceils, and TGF-β1and IL-10 in liver cancer tissues.

关 键 词：癌 肝细胞 白细胞介素10 转化生长因子B1 TREG细胞 

分 类 号：R735.7[医药卫生—肿瘤]