尼莫地平对大鼠牙髓干细胞分化后DMP-1表达的影响  

Effect of Nimodipine on DMP-1 expression in the odontogenic differentiation of rat dental pulp stem cells

作　　者：琚燕琴[1] 葛剑平[1] 黄晓峰[1] 许多[1] 蒋承智[1] 赵守亮[1] 

机构地区：[1]同济大学附属口腔医院牙体牙髓科,口腔生物医学及转化医学实验室,上海200072

出　　处：《北京口腔医学》2014年第2期75-78,共4页Beijing Journal of Stomatology

基　　金：国家自然科学基金(81070825;81170951);上海市科学技术委员会医学引导项目(10411964600)

摘　　要：目的观察尼莫地平对牙本质基质蛋白-1在大鼠牙髓干细胞分化前后基因表达的影响。方法通过特殊培养液诱导大鼠牙髓干细胞向成牙本质细胞方向分化,实验组分为正常诱导组和加入尼莫地平干扰组,在诱导后的不同时间点,1、3、5、7、10和14天,分别提取细胞的总RNA,通过Real-time PCR观察大鼠牙髓干细胞分化前后牙本质基质蛋白-1基因表达的变化,数据采用独立样本t检验。结果大鼠牙髓干细胞向成牙本质细胞方向分化后,1、3天的牙本质基质蛋白-1表达没有明显变化(P>0.05);5天变化较明显,差异有统计学意义(P<0.05);5、7、10和14天随着时间增加牙本质基质蛋白-1基因表达明显增高。加入尼莫地平干扰的诱导分化组和正常诱导组比较,同一时间点,牙本质基质蛋白-1的表达量减少(P<0.05)。结论尼莫地平可减少牙本质基质蛋白-1在大鼠牙髓干细胞向成牙本质细胞方向分化后的表达,推测L型钙离子通道可能在牙髓干细胞的分化中起重要的作用。Objective To examine the effects of nimodipine on dental matrix protein-1 （ DMP-1 ） expression during the odontogenic differentiation of rat dental pulp stem cells. Methods The rat dental pulp stem cells were differentiated into odontoblast-like cells in the odontogenic induction medium with or without nimodipine. Total RNA was extracted at day1,3,5,7,10,14 ,respectively after induction. The mRNA level of DMP-1 was detected by real-time PCR. The data were analyzed by independent-samples t-test. Results There was no significant difference between the expression level of the DMP-1 at day 1 and day 3 after the differentiation, but the difference was significant after day 5. The DMP-1 expression increased with time from day 5 to day 14. There was a significant difference between the group treated with nimodipine and the control group. Conclusion Nimodipine may reduce the DMP-1 mRNA expression after the odontogenic differentiation of rat dental pulp stem cells. It can be speculated that L-type calcium channel may be involved in the odontogenic differentiation of rat dental pulp stem ceils.

关 键 词：牙髓干细胞 L型钙离子通道 成牙本质细胞方向分化 牙本质基质蛋白-1 

分 类 号：R780.2[医药卫生—口腔医学]