低氧条件下Ezrin蛋白对乳腺癌细胞增殖和黏附能力影响的研究  

Study on the effect of Ezrin protein on proliferative ability and adhesive ability of breast cancer cells under hypoxia

作　　者：苏占海[1] 刘永年[2] 李斌[3] 王荣华[4] 乔金婉 解亚丽[6] 

机构地区：[1]青海大学医学院基础医学部,青海西宁810001 [2]青海大学病理生理学教研室 [3]青海大学流行病学教研室 [4]青海大学附属医院肿瘤放疗科 [5]青海大学生物教研室 [6]青海大学基础医学研究中心

出　　处：《中国妇幼保健》2014年第16期2602-2605,共4页Maternal and Child Health Care of China

摘　　要：目的:研究低氧条件下,Ezrin蛋白对乳腺癌细胞增殖、黏附能力及细胞骨架表达的调节作用。方法:用MTT方法检测常氧或低氧条件下,Ezrin对乳腺癌原代细胞增殖、黏附、侵袭的影响;免疫荧光染色法检测常氧或低氧条件下Ezrin对乳腺癌细胞骨架、细胞形态及细胞表面微绒毛表达的影响。结果:常氧或低氧条件下,Ezrin蛋白对乳腺癌细胞增殖均有抑制能力(P常氧<0.05,P低氧<0.05)。黏附能力检测结果显示,在常氧条件下Ezrin蛋白对乳腺癌细胞黏附能力没有明显影响(P常氧>0.05),但是低氧条件下对乳腺癌细胞黏附能力有增强作用(P低氧<0.05)。在对细胞骨架、细胞形态变化及细胞微绒毛表达实验中,观察到Ezrin在低氧条件下可加强细胞表面微绒毛表达及细胞形态变化。结论:低氧条件下,Ezrin蛋白对乳腺癌细胞的黏附能力有影响,对细胞骨架表达、细胞形态改变和细胞表面微绒毛表达的调节作用较为显著。Objective： To research the regulatory effect of Ezrin protein on proliferative ability and adhesive ability of breast cancer cells and cytoskeleton remodeling under hypoxia. Methods： MTT assay was used to analyze the effect of Ezrin protein on cell proliferation,adhesion and invasion of breast cancer primary cells under normoxia and hypoxia; immunofluorescent staining was performed to detect the effect of Ezrin protein on breast cancer cytoskeleton,cellular morphology and microvilli expression on the cell surface under normoxia and hypoxia. Results： Ezrin protein had inhibiting effect on proliferation of breast cancer cells under normoxia and hypoxia（ Pnormoxia 0. 05,Phypoxia 0. 05）. The results of adhesive ability test showed that the impact of Ezrin protein on adhesive ability of breast cancer cells under normoxia was not significant（ Pnormoxia 0. 05）,but the impact of Ezrin protein on adhesive ability of breast cancer cells under hypoxia strengthened（ Phypoxia 0. 05）. Ezrin protein could enhance microvilli expression on the cell surface and cellular morphological change under hypoxia. Conclusion： Under hypoxia,Ezrin protein can enhance the adhesive ability of breast cancer cells,and its regulatory effect on cytoskeleton remodeling,morphological change and microvilli expression of breast cancer cells are significant.

关 键 词：EZRIN蛋白 低氧 乳腺癌细胞增殖 细胞黏附侵袭 

分 类 号：R737.9[医药卫生—肿瘤]