穗花杉双黄酮对急性肝损伤大鼠炎症相关因子的影响  

Effect of Amentoflavone on Inflammatory Factors in Rats with Acute Liver Injury

作　　者：黄振青[1] 韦燕飞[1] 刘雪梅[1] 段雪琳[1] 赵铁建[1] 

机构地区：[1]广西中医药大学,南宁530001

出　　处：《中国实验方剂学杂志》2014年第11期147-150,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究穗花杉双黄酮(ATF)对四氯化碳(CCl4)诱导急性肝损伤大鼠炎症相关因子的影响。方法:将健康雄性SD大鼠随机分为正常对照组,损伤模型组,阳性对照组,穗花杉双黄酮低、中、高剂量组(15,30,60 mg·kg-1),连续给药7d,末次灌胃给药1 h后,腹腔注射CCl4原液建立急性肝损伤大鼠模型,采用ELISA法分别检测各组大鼠血清肿瘤坏死因子-α(TNF-α),转化生长因子β1(TGF-β1),白介素1β(IL-1β),白细胞介素6(IL-6)的含量和肝组织核因子-κB(NF-κB),磷酸化抑制蛋白(nuclear factor kappa B inhibitor protein,IκB-α)的水平,采用鲎试剂终点显色法检测大鼠血浆内毒素(LPS)水平,Real time PCR法检测肝组织TNF-α和诱导型一氧化氮合酶(iNOS)基因的表达。结果:与正常组比较,模型组大鼠血清TNF-α,TGF-β1,IL-1β,IL-6的含量显著升高(P<0.01),大鼠血浆内毒素水平明显提高(P<0.01),肝组织NF-kB和磷酸化IκB-α的水平也明显升高(P<0.01),肝组织TNF-α和iNOS基因的表达也显著上调(P<0.01)。与模型组比较,ATF能显著降低血清TNF-α,TGF-β1,IL-1β,IL-6的含量(P<0.05或P<0.01),降低肝组织NF-κB和磷酸化IκB-α的水平,且能显著降低LPS水平,下调肝组织TNF-α和iNOS基因的表达(P<0.05或P<0.01)。结论:穗花杉双黄酮对四氯化碳致急性肝损伤大鼠具有明显的保护作用,其机制可能通过抑制内毒素引起的Kupffer细胞激活,进而抑制下游核因子NF-κB的激活,减少炎症细胞因子的释放。Objective： To investigate the effects of amentoflavone （ATF） on inflammatory factors in rats with acute liver injury. Method： The healthy adult male Sprague-Dawley （SD） rats were randomly divided into six groups, control group, model group, positive control group, ATF low-dose, middle-dose and high-dose group （ 15, 30, 60 mg ·kg^-1 ） , respectively. The experimental rats were administrated with drugs by ig for 7 days, one hours after the last administration, the experimental rats were injected carbon tetrachloride to copy the acute liver injury model except control group. The contents of tumor necrosis factor-α （TNF-α）, transforming growth factor beta （TGF-1β） , interleukin 1β （IL-1β） , interleukin-6 （IL-6） were investigated by ELISA. The phosphor-IκB-α level and nuclear factor κB （NF-κB） nuclear translocation in hepatic tissue were measured by ELISA. The plasma endotoxin （LPS） level was measured by limulus amebocyte lysate （LAL） chromogenic endpoint assay. The expression of TNF-α mRNA and induced nitric oxide synthase （iNOS） mRNA in hepatical tissues in rats were detected by Real time PCR. Result： Compared with the normal control group, the contents of TNF-α, TGF-1β, IL-1β and IL-6 in the serum of the model group rats were obviously increased （P 〈0. 01 ） , and the level of plasmaendotoxin was obviously increased （P 〈 0. O1 ） , the phosphor-IκB-α level and NF-κB nuclear translocation were obviously increased, the expression of TNF-α mRNA and iNOS mRNA were up-regulated （both P 〈 0.01 ）. Compared with the model group, the contents of TNF-α, TGF-β1, IL-1β, IL-6 were obviously decreased （P 〈 0.05 or P 〈 0.01 ） , The phosphor-IκB-α level and NF-κB nuclear translocation were decreased （P 〈 0.05 or P 〈 0.01）, and the level of plasma endotoxin was obviously decreased, the expression of TNF-α mRNA and iNOS mRNA in hepatical tissues in rats were down-regulated （P 〈 0.05 or P 〈 0.01 ）. Conclusion ： ATF cou

关 键 词：穗花杉双黄酮 四氯化碳 急性肝损伤 炎症因子 

分 类 号：R285.5[医药卫生—中药学]