双歧杆菌四联活菌片联合芪倍合剂对溃疡性结肠炎患者T细胞亚群及Fas/FasL系统表达的调节  

Tetralogy of viable tablets combined with Qibei mixture on ulcerative colitis patients with T cell subsets and the expression of Fas/FasL system

作　　者：郭晓鹤[1] 张彩凤[1] 夏永华[2] 朱艳丽[1] 秦咏梅[1] 韩宇[1] 

机构地区：[1]新乡医学院第一附属医院消化科,河南新乡453100 [2]新乡医学院第一附属医院皮肤科,河南新乡453100

出　　处：《重庆医学》2014年第14期1684-1686,共3页Chongqing medicine

基　　金：河南省教育厅科研基金资助项目(20073200143)

摘　　要：目的探讨双歧杆菌四联活菌片(以下简称四联活菌片)联合芪倍合剂治疗溃疡性结肠炎的临床疗效及对免疫系统的调节作用。方法应用流式细胞仪和ELISA法检测四联活菌片联合芪倍合剂治疗溃疡性结肠炎前后T细胞亚群及外周血淋巴细胞Fas及FasL的表达水平,并与四联活菌片及芪倍合剂单一治疗进行比较。结果四联活菌片联合芪倍合剂治疗(第一组)总有效率为92.50%,高于四联活菌片单独治疗(第二组)(72.50%)(P<0.01)。各组治疗前外周血T细胞(CD3+)、辅助性T细胞(CD3+CD4+)、抑制性T细胞(CD3+CD8+)均高于健康对照组(P<0.01),CD4+/CD8+值升高(P<0.05),Fas表达明显升高(P<0.05)。治疗后CD3+、CD3+CD4+、CD3+CD8+较治疗前下降(P<0.05),CD4+/CD8+值降低(P<0.05),Fas明显下降(P<0.01)。结论四联活菌片可通过逆转外周血淋巴细胞的Fas/FasL系统表达异常,诱导淋巴细胞的正常凋亡,与中药芪倍合剂合用起协同作用。Objective To estimate the clinical efficacy and immunomodulatory effect of tetralogy of viable tablets combined with Qibei mixture .Methods Flow aftometry and enzyme linked immunosorbent assay （ELISA） were performed to detect T cell subsets and expression level of Fas and FasL respectively .Results The response rate was significantly higher in patients treated with tet‐ralogy of viable tablets combined with Qibei mixture than in those with tetralogy of viable tablets alone （92 .50% vs .72 .50% ,P〈0 .01） ,before the treatment ,patients showed a higher percentage of CD3＋ cells ,CD3＋CD4＋ cells and CD3＋CD8＋ cells in peripheral blood（P〈0 .01） ,weaker expression of Fas（P〈0 .01） ,but a higher CD4＋ /CD8＋ ratio（P〈0 .01）compared with the controls .The treatment with Qibei mixture and tetralogy of viable tablets alone or in combination all induced an decrease in the percentage of CD3＋cells ,CD3＋CD4＋cells and CD3＋CD8＋ cells in peripheral blood（P〈0 .05）and in the expression of Fas（P〈0 .01） ,but an de‐crease in CD4＋ /CD8＋ ratio（P〈0 .05） .Conclusion Tetralogy of viable tablets may induce the normal apoptosis in lymphocytes via reversing the abnomality in the expression of Fas/FasL by peripheral blood lymphocytes ,and the effect of tetralogy of viable tablets may be enhanced by Qibei mixture .

关 键 词：结肠炎 溃疡性 T淋巴细胞亚群 四联活菌片 芪倍合剂 FAS FasL系统 

分 类 号：R574.62[医药卫生—消化系统]