血浆检测microRNA-125a-3p、IGF-2在监测NSCLC侵袭转移中的价值  

The value of detecting plasma microRNA-125a-3p,IGF-2 on monitoring invasion and metastasis in NSCLC

作　　者：张洪川[1] 徐艳梅[1] 周璞[1] 孙建国[1] 陈正堂[1] 

机构地区：[1]第三军医大学附属新桥医院全军肿瘤研究所,重庆400037

出　　处：《重庆医学》2014年第14期1700-1702,1706,共4页Chongqing medicine

基　　金：军队临床高新技术重大基金资助项目(2010gxjs070)

摘　　要：目的探讨血浆检测microRNA-125a-3p(miR-125a-3p)、胰岛素样生长因子2(IGF-2)在监测非小细胞肺癌(NSCLC)侵袭转移中的价值,研究二者表达之间的相关性。方法应用实时定量-PCR(qRT-PCR)检测方法及2-△△CT分析法检测20例健康者、73例NSCLC患者血浆中miR-125a-3p水平,酶联免疫吸附实验(ELISA)检测血浆中IGF-2水平。结果Ⅲ/Ⅵ期miR-125a-3p水平较Ⅰ/Ⅱ期、健康组低(P=0.001、P=0.005);Ⅰ/Ⅱ期与健康组比较差异无统计学意义(P=0.776);miR-125a-3p表达与淋巴结转移相关,有淋巴结转移组呈低表达(P=0.003)。Ⅰ/Ⅱ期、Ⅲ/Ⅳ期IGF-2水平较健康组高表达(P=0.036、P=0.011);Ⅰ/Ⅱ期与Ⅲ/Ⅳ期比较差异无统计学意义(P=0.451);IGF-2水平与淋巴结转移相关,有淋巴结转移组呈高表达(P=0.037)。血浆中miR-125a-3p水平与IGF-2水平呈负相关(r=-0.280,P=0.007)。结论血浆中miR-125a-3p低表达、IGF-2高表达可能与NSCLC的侵袭转移相关,miR-125a-3p可能对IGF-2起负调控作用。Objective To investigate the value of detecting plasma microRNA‐125a‐3p（miRNA‐125a‐3p） ,IGF‐2 on monitoring invasion and metastasis in NSCLC ,and to study the correlation between miR‐125a‐3p and IGF‐2 .Methods miR‐125a‐3p transcripts of 20 controls ,73 NSCLC were performed in plasma by quantitative reverse transcription‐polymerase chain reaction（qRT‐PCR） and PCR data was analyzed by the 2‐ΔΔCT method .The expression of IGF‐2 in plasma was detected by ELISA .Results The expression of miR‐125a‐3p in stage Ⅲ /Ⅳ was lower than stage Ⅰ/Ⅱ and the controls（P=0 .001 ,P=0 .005） .There was no statistical differ‐ence between the stage Ⅰ /Ⅱ patients and the controls（P=0 .776） .The expression of miR‐125a‐3p was related with lymph node metastas ,lower expression in positive lymph node metastasis （P=0 .003） .The expression of IGF‐2 in stage Ⅰ /Ⅱ 、stage Ⅲ /Ⅳ was higher than the controls（P=0 .036 ,P=0 .011） .There was no statistical difference between the stageⅠ/Ⅱ and stage Ⅲ/Ⅳ （P=0 .451） . The expression of IGF‐2 was related with lymph node metastas ,higher expression in positive lymph node metastasis （P=0 .037） .The re‐sults showed a negative correlation between miR‐125a‐3p expression and IGF‐2 in plasma（r= -0 .280 ,P=0 .007） .Conclusion Low ex‐pression of miR‐125a‐3p and high expression of IGF‐2 in plasma may play a role in invasion and metastasis of NSCLC .miR‐125a‐3p may play a negative regulatory role on IGF‐2 .

关 键 词：癌 非小细胞肺 miR-125a-3p IGF-2 转移 

分 类 号：R734.2[医药卫生—肿瘤]