辛二酰苯胺异羟肟酸对鼻咽癌细胞回复引导半胱氨酸丰富蛋白含kazal基元以及基质金属蛋白酶9表达与活性的影响  

Impact of Suberoylanilide Hydroxamic Acid on Reversion- inducing- cysteine- rich Protein with Kazal Motifs and Matrix Metalloproteinases 9 Expression and Activity in Nasopharyngeal Carcinoma Cells

作　　者：王柏琦[1,2] 陈艳华[1] 蒋丽琴[1] 程爱兰[2] 

机构地区：[1]南华大学附属第二医院,湖南省衡阳市421001 [2]南华大学肿瘤研究所

出　　处：《实用心脑肺血管病杂志》2014年第6期42-44,共3页Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease

基　　金：国家自然科学基金(81372894)

摘　　要：目的探讨辛二酰苯胺异羟肟酸(suberoylanilide hydroxamic acid,SAHA)对鼻咽癌细胞回复引导半胱氨酸丰富蛋白含kazal基元(reversion-inducing-cysteine-rich protein with kazal motifs,RECK)以及基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)表达与活性的影响。方法体外培养鼻咽癌细胞系CNE-1,用0、0.1、1、5μmol/L SAHA处理后,采用蛋白质印迹法(Western blot)和Real-Time PCR(RT-PCR)分别检测RECK,MMP-9蛋白和mRNA表达;MTT法检测CNE-1细胞的增殖。同时采用明胶酶谱实验观察SAHA处理后MMP-9酶活性变化。结果随着SAHA浓度的增加,RECK蛋白和mRNA的表达显著增高(P<0.05);随着SAHA浓度的增加,MMP-9蛋白和mRNA的表达显著降低(P<0.05);CNE-1细胞随着SAHA浓度的增加,存活率逐渐降低(P<0.05)。明胶酶谱实验显示,随着SAHA浓度的增加,MMP-9酶活性逐渐降低。结论 SAHA可能通过上调RECK基因的表达,抑制MMP-9的表达与活性而发挥对CNE-1细胞生长抑制作用。Objective To investigate the impact of suberoylanilide hydroxamic acid（ SAHA） on reversion- inducing- cysteine- rich protein with kazal motifs（ RECK） and matrix metalloproteinase- 9（ MMP- 9） expression and activity in nasopharyngeal carcinoma cells. Methods Nasopharyngeal carcinoma cell line CNE- 1 was cultured in vitro,and stimulated by 0,0. 1,1 and 5 μmol / L SAHA,expression of protein and mRNA of RECK and MMP- 9 were detected by Western blot and Real- time PCR（ RT- PCR） respectively; cell proliferation was assessed by MTT assay. Expression and enzymic activity of MMP- 9 were detected by Gelatin zymography assay. Results With increasing concentrations of SAHA,expression of protein and mRNA of RECK were significantly higher（ P〈0. 05）; with increasing concentrations of SAHA,expression of protein and mRNA of MMP- 9 were significantly lower（ P〈0. 05）; with increasing concentrations of SAHA,the survival of CNE- 1 and enzymic activity of MMP- 9 were decreased（ P〈0. 05）. Conclusion SAHA may increase expression of RECK gene and restrain activity of MMP- 9,and inhibit CNE- 1 cells growth.

关 键 词：鼻咽肿瘤 基质金属蛋白酶9 回复引导半胱氨酸丰富蛋白含kazal基元 辛二酰苯胺异羟肟酸 

分 类 号：R739.62[医药卫生—肿瘤]