HPLC-ELSD测定油菜蜂花粉中6种脂肪酸  

Determination of Six Kinds of Fatty Acids in Rape Bee Pollen by HPLC-ELSD

作　　者：谢元勋[1] 何厚洪 盛卫国 叶剑锋 吴健 王如伟[1,2] 

机构地区：[1]浙江中医药大学药学院,杭州310053 [2]浙江现代中药与天然药物研究院有限公司,浙江省中药制药技术重点实验室,杭州310052

出　　处：《中国现代应用药学》2014年第5期559-563,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江重点企业创新研究院项目;浙江省重大科技专项重点社会发展项目(2012C03005-5);杭州市产学研合作项目(20122331E35)

摘　　要：目的建立HPLC-ELSD法分析油菜蜂花粉中6种脂肪酸，并测定其含量。方法色谱柱Agilentn ZORBAXSB-C18（4．6mm×250mm，5μm）；流动相：甲醇-1％乙酸水梯度洗脱，流速1．2mL·min^-1，柱温25℃；检测器参数：增益：6．0，漂移管温度：30℃，载气：氮气，压力：0.3MPa。结果十四烷酸、亚麻酸、亚油酸、软脂酸、油酸、十八烷酸分离度均在1．5以上，加样回收率均位于95．3％～103．7％，相对标准偏差（n=6）分别为2．2％，2．0％，1．0％，1．4％，1．4％，2．3％。结论该方法简便、准确、重复性好，适合油菜蜂花粉原药材中高含量脂肪酸成分的分析测定。OBJECTIVE An high-performance liquid chromatographic method with an evaporative light-scattering detector has been developed for the separation and determination of six kinds of fatty acids in rape bee pollen. METHODS An Agilent ZORBAX SB-C18 analytical column（4.6 mm×250mm, 5 μm） with methanol-acetic acid aqueous solution as mobile phase at a flow rate of 1.2 mL·min^-1 were applied. The column temperature was set at 25 ℃. The parameters of ELSD were as follows： the plus was 6.0; the temperature of the drift tube was 30 ℃, the carrier gas pressure was 0.3 MPa. RESULTS The separation degree of stearic, linolenic, linoleic, palm, oleic and myristic acids were above 1.5, and their recoveries ranged from 95.3% to 103.7%, the relative standard deviations（n=6） were 2.2%, 2.0%, 1.0%, 1.4%, 1.4%, 2.3%. CONCLUSION The method is simple, accurate and reproducible, it＇s suitable for the simultaneous determination of the six high fatty acids in rape bee pollen.

关 键 词：油菜蜂花粉 脂肪酸 高效液相色谱-蒸发光散射检测法 含量测定 

分 类 号：R917.101[医药卫生—药物分析学]