一株筛选自高寒草地木聚糖酶产生真菌的鉴定及酶学特性研究  

A Study on Identification and Enzyme Characteristics of a Xylanase Producing Strain Isolated and Selected from the Soil in Alpine Meadow

作　　者：芦光新[1] 陈秀蓉[2] 王军邦[3] 吴楚[4] 

机构地区：[1]青海大学农牧学院,西宁810016 [2]甘肃农业大学草业学院,兰州730070 [3]中国科学院地理科学与资源研究所,北京100094 [4]长江大学园艺园林学院,荆州434025

出　　处：《生物技术通报》2014年第5期162-169,共8页Biotechnology Bulletin

基　　金：国家自然科学基金项目(41261064);青海大学高层次人才基金项目(2012-QGC-9)

摘　　要：为研究一株分离、筛选自高寒草地土壤中的真菌,采用ITS-rDNA基因序列分析法、液体摇瓶发酵法和传统微生物培养法,对供试菌株的分类学地位进行了确定,并对该菌株产木聚糖酶的酶学性质及其主要生物学特性进行了测定。结果表明,经ITS-rDNA基因序列分析法鉴定,供试菌株初步确定为Saccharicola bicolor。供试菌株S.bicolor可分泌木聚糖酶,且在pH为3.0-9.5时,酶活力相对稳定,表明S.bicolor所分泌的木聚糖酶具有一定的耐受碱性条件的能力。供试菌株S.bicolor在温度为35℃时生长较快,对5种碳源利用情况的先后顺序为:麦芽糖>淀粉>蔗糖>糊精>葡萄糖,对5种氮源利用情况的先后顺序为:蛋白质>磷酸铵>硫酸铵>硝酸钠>尿素。A fungus strain has been isolated and selected from the soil in alpine meadow. Using gene sequences of rDNA-ITS analysis, flask liquid fermentation and traditional culture methods. Taxonomic position of strains were identificate, and xylanase characteristics and its main biological characteristics were also been investigated. The results showed that by rDNA-ITS sequence analysis, the fungus was identified as Saccharicola bicolor. S. bicolor can secrete xylanase, its enzyme activity is relatively stable under the condition, i.e., pH3.0-9.5, showed that xylanase secreted by S. bicolor have certain tolerance ability of alkaline conditions. The fungus grew rapidly under the condition, i.e., 35 ℃, showing different requirements for carbon and nitrogen resources, i.e., the order of carbon resources is maltose, starch, sucrose, dextrin, and glucose ; the order of nitrogen resources is proteins, amine phosphate, amine sulfate, sodium nitrate, and urea.

关 键 词：高寒草地 木聚糖酶 ITS-RDNA pH稳定性 生物学特性 

分 类 号：Q936[生物学—微生物学]