海洋来源放线菌Streptomyces parvulus OUCMDZ-2554产放线菌素D的发酵条件优化  

Optimization of fermentation conditions for production of actinomycin D by Marine-derived Streptomyces parvulus OUCMDZ-2554

作　　者：王聪[1] 刘培培[1] 王乂[1] 孙坤来[1] 贾海健[1] 朱伟明[1] 

机构地区：[1]中国海洋大学海洋药物教育部重点实验室,医药学院,山东青岛266003

出　　处：《中国海洋药物》2014年第3期34-42,共9页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金(21172204);国家重点基础研究发展计划(2010CB833804);国家高技术研究发展计划(2013AA092901);国家海洋局公益性项目(2010418022-3)资助

摘　　要：目的对海洋来源放线菌Streptomyces parvulus OUCMDZ-2554产放线菌素D的发酵条件进行优化,提高放线菌素D的产量。方法对培养基的组成、种龄、装液量、pH、盐度和发酵时间等条件的研究,通过单因素和正交试验,选择最优发酵条件;并通过波谱分析及其理化性质确定放线菌素D结构。结果最佳培养基为:K2HPO41.5g、MgSO40.5g、酵母浸膏5g、可溶性淀粉22.5g、陈海水1 000mL;最佳发酵条件:装液量150/500mL(体积分数)、种龄4d、盐度3%、起始pH=7.5、摇床(180rpm)发酵12d。结论以最佳发酵条件发酵,优化后放线菌素D的产量为优化前的3.6倍,达到364mg/L。Objective To improve the productivity of actinomycin D from marine-derived actinomycete Strepto- rnyces parvulus OUCMDZ-2554 by optimization of the fermentation conditions. Methods The optimal composition of the medium, the inoculum age and size, temperature, liquid volume, pH, salinity and cultivation time were investigated by single-factor experiment and the orthogonal matrix methods. The structure of actinomycin D was identified by means of spectroscopic analysis and physicochemical properties. Results The optimum culturing medium was as following. K2HPO4 1.5 g, MgSO4 0. 5 g, yeast extract 5 g, soluble starch 22. 5 g, sea water 1000 mL. And the best fermentation conditions were 150 mL of culture medium in 500 mL. conical flask, 4 d old of inoculum age and 3% of salinity, pH=7.5, and shaking in an orbital shaker （180 rpm） for 12 d. Conclusion The best yield （364 mg/L） of actinomycin D is obtained under the optimal fermentation conditions, which is 3.6-fold high as the original conditiorL

关 键 词：放线菌素D 发酵优化 海洋放线菌 STREPTOMYCES parvulus 

分 类 号：R931[医药卫生—生药学]