深低温保存人羊膜移植兔眼结膜重建后的组织学改变  

The Historical Changes after Preserved Human Amnotic Membrane Transplantation for Conjunctival Reconstruction of Rabbits Eyes

作　　者：卢蓉[1] 刘金陵[1] 张洁[1] 郑湖玲[1] 袁钊辉[1] 林建贤[1] 

机构地区：[1]中山医科大学中山眼科中心,广州510060

出　　处：《眼科学报》2000年第4期224-227,共4页Eye Science

摘　　要：目的:探讨深低温保存羊膜移植修复兔眼结膜缺损术后的组织学变化。方法:取12只新西兰白兔,做右眼结膜缺损模型,然后进行深低温保存的羊膜移植。在术后1周,3周,5周,12周分别处死动物取材,取术区标本(包括植片和植床)进行组织学检查,包括HE染色和PAS染色,并对术后12周的术区标本进行透射电镜检查。结果:所有术眼外观均生长良好,无植片脱落、溶解、瘢痕化或睑球粘连。术后1周,羊膜植片的死亡上皮细胞覆盖基底膜;术后3周植片原有的上皮被小而圆的结膜上皮细胞替代,有杯状细胞分化,复层或单层分布,羊膜开始溶解;术后5周结膜上皮细胞分化良好,术后12周羊膜溶解消失。透射电镜所见:术后12周上皮细胞分层排列良好,细胞内含丰富的线粒体和粗面内质网,表层细胞有大量微绒毛,细胞之间有丰富的桥粒,基底膜完整,细胞与基底膜之间见半桥粒结构。结论:深低温保存的羊膜移植修复结膜术后,不仅外观良好,而且组织结构与正常结膜一致。因此,深低温保存羊膜是一种有应用价值的结膜替代物。眼科学报2000;16:224～227。Purpose: To investigate the histological changes after preserved human amnotic membrane transplantation of rabbit eyes. Methods : The conjunctiva of 12 New Zealand albino rabbits' right eyes were excised partly. Then the preserved human amniotic membrane were transplanted on the sclera. Eyes were enucleated at 1 week, 3 weeks, 5 weeks and 12 weeks (n = 3 for each) after surgery, and they were examined histologically with staining of HE, PAS, and transmission electronic microscopy. Results: At 1 week after transplantation, the human amniotic membrane firmly attached on the sclera, with the dead epithelial cells remaining on its surface. At 3 weeks, conjuncti-val epithelization was completed on the human amniotic membrane. Abundant PAS - positive goblet cells were recognized. The amnion began resolve. At 12 weeks, the amnion dissolved. On transmission electronic microscopy, the cell polarity is prominent. The epithelial cells contained a large number of mitochondria and endoplasmic reticulum. Microvil-li were pronounced on the apical cell membrane. There are many intracellular desmo-somes and hemidesmosomes. Conclusion: Preserved human amniotic membrane is a valuable conjunctival replacement material. After transplantation, the histology of new tissue is as same as normal conjunctive. Eye Science 2000; 16: 224-227.

关 键 词：人羊膜 移植 结膜重建 组织学改变 

分 类 号：R779.6[医药卫生—眼科]