乳腺癌患者骨髓微小转移灶的检测及临床意义  

Detection of micrometastasis in the bone marrow of patients with breast cancer

作　　者：臧静[1] 陈龙邦[1] 王靖华[1] CHU Xiao-yuan 褚晓源[1] 张群[1] 龚涌灵[1] 

机构地区：[1]南京军区南京总医院肿瘤科 [2]Department of Oncology Jinling Hospital

出　　处：《医学研究生学报》2001年第1期33-34,共2页Journal of Medical Postgraduates

摘　　要：目的：检测乳腺癌患者骨髓微小转移灶，分析其与临床病期及远处转移的关系，并探讨其临床意义。　 方法：采用上皮膜抗原(EMA)单克隆抗体为探针，用免疫组化ABC法检测乳腺癌患者骨髓中EMA表达阳性细胞。结果：58例乳腺癌患者有33例骨髓中找到EMA表达阳性细胞，阳性率为57%；晚期患者阳性细胞检出率显著增高；术后复发及有骨转移的患者较无复发及骨转移的患者阳性细胞检出率高；该方法与骨髓常规涂片检查比较，其阳性细胞检出率有显著差异。 　结论：骨髓中EMA表达阳性细胞的检测可作为评价乳腺癌患者病情、了解有无复发及骨转移的重要指标之一。Objectives: To study the relationship between bone marrow micrometastasis of breast cancer and clinical stage and metastasis. Methods:Bone marrow epithelial membrane antigen(EMA) positive cells from patients with breast cancer were measured by immunocytochemistry using an antibody probe. Results: EMA positive cells were detected in the bone marrow of 33 patients(57%), the positive rates correlated with the stage of patients, and the recurrence rate in patients with bone metastasis were higher than those without metastasis. There was significant difference between screen smear and EMA method in detecting bone marrow micrometastasis. Conclusions: The method of detecting EMA positive cells is important in evaluating the stage of patients with breast cancer and to know whether patients have recurrence and bone metastasis.

关 键 词：乳腺癌 免疫组化 骨髓微小转移灶 

分 类 号：R737.903[医药卫生—肿瘤]