IFN-α对大鼠肝纤维化间质胶原酶基因表达的调控  

Effect of interferon-alpha on interstitial collagenase gene expression in rat liver fibrosis

作　　者：王宇[1] 高毅[1] 俞金龙[1] 黄宇琦 蒋晓青[3] 

机构地区：[1]中国人民解放军第一军医大学珠江医院肝胆外科,广东省广州市510282 [2]中国人民懈放军第一军医大学南方医院肝胆外科 [3]中国人民解放军第一军医大学南方医院麻醉科

出　　处：《世界华人消化杂志》2001年第1期20-23,共4页World Chinese Journal of Digestology

基　　金：广东省自然科学基金 No.960362

摘　　要：目的了解α-干扰素(IFN-α)对大鼠实验性肝纤维化的疗效及其在治疗过程中对肝内间质胶原酶基因表达水平变化的影响。方法建立CCl_4中毒性大鼠肝纤维化模型(n=64),于肝纤维化早中期以IFN-α_(2b)治疗6wk,采用逆转录定量PCR方法测定肝内MMP-13在不同治疗时间的基因表达变化情况。结果治疗组较对照组肝纤维化程度明显减轻,大鼠肝组织中MMP-13在治疗组不同治疗时间(2,4,6wk)的基因表达相对值较对照组增强(分别为0.77±0.12vs0.83±0.13;1.01±0.08vs0.80±0.09;1.73±0.09vs0.76±0.17;2.16±0.12vs0.78±0.13,P<0.01),在治疗末期达到最高峰。结论 IFN-α对大鼠肝纤维化具有良好的疗效可能与促进MMP-13的基因表达有关。AIM To investigate therapeutic effect of interferon-alpha (IFN-α) on rat experimental liver fibrosis and the alteration of Interstitial collagenase (MMP-13 in rat) gene expression level in the liver during the treatment. METHODS The carbon tetrachloride-induced hepatic fibrosis rat models ( n = 64) were used. At the early or Intermediate stage of fibrosis, we used IFN-α2b for 6 weeks to treat the disease. MMP-13 mRNA expression level in the liver was measured by quantitative analysis of reverse transcription polymerase chain reaction (RT-PCR) during the treatment of experimental liver fibrosis. RESULTS Compared with the control group, the fibrosis degree in the IFN-α treatment group was remarkably reduced and MMP-13 gene expression relative level in the treatment group (2, 4, 6 wk) significantly increased in different time of treatment (0.77 ± 0.12 vs 0.83 ± 0.13; 1.01±0.08 vs 0.80±0.09; 1.73±0.09 vs 0.76±0.17; 2.16±0.12 vs 0.78±0.13, respectively, P<0.01). The expression rate peaked at the end of the treatment. CONCLUSION The good curative effect of IFN-α on liver fibrosis may be related to enhancement of MMP-13 gene expression.

关 键 词：肝硬化 金属蛋白酶类 干扰素Α 基因表达 聚合酶 链反应 细胞外基质 

分 类 号：R575.2[医药卫生—消化系统]