培养基及琼脂浓度对小麦花药出愈率的影响  

The Effect of the Agar Concentration in Different Medium on the Initiating Rate of the Anther Culture in Wheat

作　　者：傅晓艺[1] 付艺伟 刘桂茹[3] 何明琦[1] 史占良[1] 

机构地区：[1]石家庄市农林科学研究院,石家庄050041 [2]景县中学,河北景县053500 [3]河北农业大学,河北保定071000

出　　处：《中国农学通报》2014年第15期46-49,共4页Chinese Agricultural Science Bulletin

基　　金：国家科技支撑计划(2011BAD35B03);石麦15号小麦新品种(11220121D)

摘　　要：为了探讨不同小麦品种（系）的抗旱性,通过在N6和C17培养基中加入不同浓度的琼脂,选用抗旱性不同的5个冬小麦品种进行花药培养,调查花药出愈率。结果表明,供试的5个冬小麦品种在N6和C17培养基（6 g/L）下出愈率相当,随琼脂浓度的升高（6-18 g/L）,小麦花药出愈率有降低的趋势,可以用C17培养基来间接判断小麦品种的抗旱能力。To explore the drought resistance in wheat varieties（lines）, different concentrations of agar was added into C17 and N6 medium, 5 winter wheat cultivars for anther culturing. The results showed that, the callus induction rate was considerable under N6 and C17 medium（6 g/L）, with the agar concentration increased（6-18 g/L）, wheat anther callus tends to decrease, C17 medium can indirectly judge wheat drought resistance.

关 键 词：琼脂 小麦 花药 出愈率 

分 类 号：S512.11[农业科学—作物学]