DADS下调Cathepsin D抑制人胃癌MGC803细胞侵袭转移  

Down-regulated Cathepsin D Inhibits Migration and Invasion in Human Gastric Cancer MGC803 Cells Induced by Diallyl Disulfide

作　　者：殷耒兰 陈琳[1] 苏菊[1] 姜浩[1] 苏琦[2] 黄卫国[2] 李跃华[1] 唐三元[1] 

机构地区：[1]湖南省胃癌研究中心南华大学附属第一医院肿瘤内科,湖南衡阳421001 [2]南华大学医学院肿瘤研究所

出　　处：《中南医学科学杂志》2014年第2期129-134,共6页Medical Science Journal of Central South China

基　　金：湖南省自然科学衡阳联合基金(10JJ9017)

摘　　要：目的研究二烯丙基二硫(DADS)是否通过下调组织蛋白酶D(Cathepsin D)的表达抑制人胃癌MGC803细胞侵袭转移。方法 RT-PCR和Western blot检测DADS及干扰Cath-D对人胃癌MGC803细胞Cath-D mRNA与蛋白表达变化;细胞划痕实验和Transwell实验分别检测DADS及干扰Cath-D对人胃癌MGC803细胞侵袭转移能力的变化。结果 DADS作用人胃癌MGC803细胞24 h后,Cath-D mRNA与蛋白表达水平明显下降(P<0.05),划痕距离明显增宽(P<0.05),穿膜细胞数明显减少(P<0.05);siRNA干扰Cath-D后,Cath-D mRNA与蛋白的表达水平明显下降(P<0.05),划痕距离明显增宽(P<0.05),穿膜细胞数明显减少(P<0.05)。结论 DADS能抑制人胃癌MGC803细胞的侵袭转移能力,其机制可能与下调Cath-D的表达有关。Objective To investigate whether diallyl disulfide （ DADS） inhibits human gastric carcinoma MGC803 cells invasion and metastasis by down-regulating the expression of Cathepsin D. Methods The mRNA and protein ex-pression level of Cath-D were detected by RT-PCR and Western blot respectively;Migration and invasion potentials were ex-amined by Wound Healing assay and transwell assay. Results Human gastric carcinoma MGC803 cells exposed to DADS30 mg/L for 24h,the mRNA and protein expression level of Cath-D were significantly decreased （P〈0. 05）,scar distance was widened （P〈0. 05）,the number of cancer cells which passed through the Matrigel coated membrane was re-duced （P〈0. 05）;after Cath-D was interfered by siRNA,the mRNA and protein expression level of Cath-D were signifi-cantly decreased （P〈0. 05）,scar distance was widened （P〈0. 05）,the number of cancer cells which passed through the Matrigel coated membrane was reduced （P〈0. 05）. Conclusions DADS can significantly inhibit the ability of migra-tion and invasion ability of human gastric carcinoma MGC803 cells and its mechanism may be related to the expression of down-regulating Cath-D.

关 键 词：胃癌 DADS 组织蛋白酶D 转移 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]