转录因子Oct-4在肺癌中的表达及对其生长、增殖的影响  

Expression of Oct-4 on lung cancer cells and its role in growth and proliferation

作　　者：雷晓芬[1] 闫磊[2] 司静琦[1] 

机构地区：[1]解放军第323医院,陕西西安710054 [2]解放军第273医院,新疆库尔勒841000

出　　处：《临床军医杂志》2014年第5期454-457,共4页Clinical Journal of Medical Officers

摘　　要：目的观察Oct-4蛋白在肺癌组织及肺癌细胞系中的表达,探讨Oct-4对肺癌细胞生长、增殖的影响。方法比较正常组织、肺癌组织,正常肺细胞系和肺癌细胞系中Oct-4的表达水平;选取高表达Oct-4的肺癌细胞系,运用siRNA技术降低Oct-4表达,通过MTT实验、BrdU掺入实验等技术检测肺癌细胞的生长、增殖,并检测PI3K分子的表达变化。结果 Oct-4在肺癌组织及肺癌细胞中表达上调,分别与正常组织及HBE比较差异有统计学意义(P<0.05);降低Oct-4表达后,细胞BrdU掺入率明显降低(P<0.05),生长速度减慢(P<0.05),PI3K水平明显降低。结论 Oct-4在肺癌组织及肺癌细胞中表达升高,并通过PI3K促进肺癌细胞生长及增殖。Objective To investigate the expression of Oct4 on lung cancer cells and its role in their growth and proliferation. Methods Lung cancer tissues and lung cancer cell lineswere selected. The expression of Oct4 was detected by Western blot. Oct- 4 gene expression was reduced by siRNA. The effect of Oct4 on growth and proliferation of lung cancer cells was measured by BrdU labeling and MTT assay, and the change in PI3K expression was detected. Results Compared with normal lung tissue and control cell group, Oct4 level significantly increased （P 〈 0.05 ）. BrdU labeling showed that incorporation efficiency of BrdU was higher in siRNA group than in control group and vector group （P 〈 0.05 ）. And we also found that after suppressing Oct-4 level, growth velocity of lung cancer cells was negative acceleration （P 〈 0.05）. Western blot displayed that the level of PI3K reduced with the decrease of Oct-4 level. Conclusion The expression of Oct4 issignificantly increased in lung cancer tissue and cancer ceils, and Oct4 promotes the growth and proliferation of lung cancer cell via PI3K pathway.

关 键 词：OCT4 PI3K 肺肿瘤 增殖 

分 类 号：R734.2[医药卫生—肿瘤]