E3泛素连接酶Iduna在非小细胞肺癌中的表达及其临床意义  

Expression and clinical significance of E3 ubiquitin ligase Iduna in non-small-cell lung cancer

作　　者：高英[1] 宋成洋 惠林萍[3] 田野[2] 田大力[2] 邱雪杉[4] 王恩华[4] 

机构地区：[1]中国医科大学附属第四医院病理科,沈阳110032 [2]中国医科大学附属第四医院胸外科,沈阳110032 [3]中国医科大学附属第四医院中心实验室,沈阳110032 [4]中国医科大学附属第一医院病理科及基础医学院病理学教研室

出　　处：《中国医师进修杂志》2014年第14期54-58,共5页Chinese Journal of Postgraduates of Medicine

基　　金：国家自然科学基金（30973502）;辽宁省科技厅科学技术计划（2012225072）

摘　　要：目的 探讨E3泛素连接酶Iduna mRNA和蛋白在非小细胞肺癌组织和癌旁肺组织中的表达及其与临床病理因素和预后的关系.方法 应用反转录聚合酶链反应（RT-PCR）、Western-Blot和免疫组织化学（EnVision）方法检测分析非小细胞肺癌组织和癌旁肺组织中Iduna mRNA和蛋白的表达,并分析其与临床病理因素和预后的关系.结果 RT-PCR及Western-Blot结果显示非小细胞肺癌组织中Iduna mRNA和蛋白表达水平分别为0.468±0.086和2.554±0.544,明显高于癌旁肺组织的0.203±0.070和1.570±0.316,差异均有统计学意义（P＜0.05）;免疫组织化学结果显示：Iduna在肺泡上皮细胞中呈阴性,在正常支气管黏膜上皮中呈阴性或弱阳性,在非小细胞肺癌组织中呈不同程度的阳性表达.Iduna的高表达率与肿瘤分化程度呈负相关（P=0.002）,与肿瘤大小（P=0.044）、TNM分期（P=0.015）、淋巴结转移（P=0.009）呈正相关,并与Ⅰ期非小细胞肺癌患者的预后有关（P=0.016）.结论 Iduna的高表达可能与非小细胞肺癌的侵袭、转移过程密切相关,有可能是提示非小细胞肺癌患者预后不良的指标.Objective To investigate the mRNA and protein expression of E3 ubiquitin ligase Iduna in non-small-cell lung cancer tissue and para-neoplastic lung tissue,and the correlation of the Iduna expression with clinicopathological factors and prognosis.Methods The expression levels of the Iduna mRNA and protein in non-small-cell lung cancer tissue and para-neoplastic lung tissue were determined by reverse transcriptase-polymerase chain reaction （RT-PCR）,Western-Blot and immunohistochemistry respectively,and the correlation of the Iduna expression with clinicopathological factors and prognosis was analyzed.Results RT-PCR and Western-Blot showed the expression levels of the Iduna mRNA and protein in non-small-cell lung cancer tissue （0.468 ± 0.086 and 2.554 ± 0.544） were significantly higher than those in para-neoplastic lung tissue （0.203 ± 0.070 and 1.570 ± 0.316）,there were statistical differences （P 〈 0.05）.Immunohistochemistry results showed that Iduna was negative expression in the alveolar epithelium cells,negative or weak positive expression in normal bronchial and positive expression in different degrees in the non-small-cell lung cancer tissue.Iduna high expression rate was negative correlation with tumor differentiation （P =0.002）,Iduna high expression rate was positive correlation with large tumor size （P =0.044）,TNM staging （P=0.015） and lymph node metastasis （P=0.009）.Iduna high expression of I stage non-small-cell lung cancer patients was correlated with poor post-operative survival （P =0.016）.Conclusions High expression of Iduna may be related to the process of invasion and metastasis of nonsmall-cell lung cancer.It is possible that Iduna serve as potential markers for predicting prognosis in nonsmall-cell lung cancer.

关 键 词：癌 非小细胞肺 肿瘤转移 预后 Iduna 

分 类 号：R734.2[医药卫生—肿瘤]