胰腺癌细胞系PC1.0、PC-1、MEK1/2仓鼠模型的建立及成瘤特性的比较  

Establishment of animal model and tumor characteristics comparative observation of pancreatic cancer cell line PC1.0、PC-1、MEK1/2 hamster pancreatic cancer

作　　者：孙洪利[1] 韩冰[1] 程新华[1] 马凯[1] 

机构地区：[1]中国医科大学盛京医院胆道血管外科,辽宁沈阳110004

出　　处：《中国现代普通外科进展》2014年第4期253-257,共5页Chinese Journal of Current Advances in General Surgery

基　　金：国家自然科学基金(30973501)

摘　　要：目的:观察不同肿瘤细胞系在仓鼠体内种植生长的差异,阐明不同胰腺癌细胞系的侵袭转移特性。方法:胰腺癌细胞系PC1.0、PC-1、MEK1/2行仓鼠胰腺原位种植、腹腔注射,建立不同的胰腺癌细胞侵袭动物模型。结果:高侵袭转移PC1.0组在肿瘤组织生长大小、腹水出现情况、肿瘤腹腔转移情况明显高于其他3组细胞;MEK1基因敲减组在胰腺种植、腹腔种植肿瘤组织生长比MEK2基因敲减组生长减小,腹膜及肝脏转移及周围脏器侵袭比MEK2基因敲减组增多。结论:PC1.0肿瘤生长旺盛且转移多;PC-1肿瘤生长一般且转移少;MEK1基因敲减肿瘤生长受抑制,但侵袭转移正常;MEK2基因敲减肿瘤生长不受抑制,但侵袭转移受抑制。Objective： To observe the difference growth of pancreatic cancer planted in hamsters. Clarfy the invade and metastasisis characteristic of pancreatic cancer. Methods： Established animal model with Pancreatic cancer cell system PC1.0; PC-1, MEK1 knowed down and MEK2 knowed down cell lines. Tumor cells was liant in animal pancreat and injected in abdomen. Results：Compare with PC1.0, the tumor grows, invasion, metastasis of MEK1 knowed down and MEK2 knowed down cell lines were restrain. MEK2 knowed down pancreatic tumor ceil displayed a weak- en invasion and metastasis ability than MEK1 knowed down pancreatic tumor cell, MEK1 knowed down pancreatic tumor cell displayed a weaken tumor grows than MEK2 knowed down pancreatic tumor cell. Conclusons： MEK1/2 gene inhibition inhibits the pancreatic cancer cell invasion and metastasis. MEK1/2 may serve as the candidate gene or potential target for the development of the new clinical pancreatic cancer drug.

关 键 词：胰腺肿瘤 PC1 0 细胞系 MEK1 2 基因敲减 仓鼠 

分 类 号：R735.9[医药卫生—肿瘤]