载脂蛋白A1与结直肠肿瘤恶性度的相关研究  

Expression of apolioprotein A1 and its correlation with the malignant degree of colorectal cancer

作　　者：胡慧慧[1] 孟倩[1] 张曼[1] 

机构地区：[1]首都医科大学附属北京世纪坛医院临床检验中心,北京100038

出　　处：《首都医科大学学报》2014年第2期243-247,共5页Journal of Capital Medical University

基　　金：北京市自然科学基金(7122086)~~

摘　　要：目的研究载脂蛋白A1(apolipoprotein A1,APOA1)在结直肠息肉、不同分级分期及远处转移的结直肠癌患者血清中的表达,旨在探讨APOA1与结直肠肿瘤恶性度的相关性。方法收集59位结直肠肿瘤患者血清,由病理学医师根据肿瘤细胞浸润及分化程度进行分期及分级,并对是否伴随远处转移进行分类。本实验采用蛋白免疫印迹法与免疫比浊法对APOA1的表达进行分析。结果 APOA1在低分化结直肠癌患者血清与息肉、高分化及中分化血清相比表达量减少(P=0.035,P=0.028,P=0.028),APOA1在结直肠息肉血清中的表达量与不同分期及远处转移的结直肠癌血清中的表达比较,差异无统计学意义(P>0.05)。结论 APOA1与结直肠肿瘤的分级存在相关性,对不同恶性度的结直肠肿瘤患者可发挥肿瘤标志物的作用。Objective To investigate the expression of apolipoprotein A1 in the serum of different stage and grade patients with colorectal polyp and cancer. We also collected the serum of patients with clinical distant metastasis of colorectal cancer, to find the relationship among apolipoprotein A1 expression and the colorectal tumor aggression and migration. Methods We collected the samples from 59 cases of colorectal tumor patients from Beijing Shijitan Hospital. The patients with colorectal cancer were classified according to the differentiation and invasion of cancer cells by pathologists. The expression of serum apolipoprotein A1 was quantitative identified by immunoturbidimetry and semi-quantitative by Western blotting. SPSS statistical analysis software was used for analyzing the results, P〈0. 05 was thought to be statistically different. Results The expression of serum apoliopoprotein A1 was lower expressed in the low differentiation of colorectal cancer compared with polyp, high differentiation and middle differentiation groups（P=0. 035, P=0. 028, P=0. 028）. Apoliopoprotein A1 was not correlated with the stage and metastases of colorectal cancer. Conclusion The serum apolipoprotein A1 was correlated with the grade of colorectal cancer. Apoliopoprotein A1 could play as a tumor marker for colorectal cancer.

关 键 词：载脂蛋白A1 结直肠肿瘤 恶性度 蛋白免疫印迹 免疫比浊法 

分 类 号：R730.3[医药卫生—肿瘤]