新疆羊狂犬病病毒的鉴定及N基因序列分析  

Identification and sequence analysis of the rabies virus N gene from sheep in Xinjiang Uygur Autonomous Region,China

作　　者：陈继章[1,2] 谷文喜[2] 钟旗[2] 舒展[3] 吕春华[3] 叶锋[2] 马晓菁[2] 易新萍[2] 

机构地区：[1]新疆农业大学动物医学院,乌鲁木齐830052 [2]新疆畜牧科学院兽医研究所,乌鲁木齐830000 [3]新疆阿勒泰地区动物疾病控制与诊断中心,阿勒泰836500

出　　处：《中国人兽共患病学报》2014年第5期494-498,共5页Chinese Journal of Zoonoses

基　　金：农业部公益性行业专项(201303035)资助~~

摘　　要：目的诊断疑似羊狂犬病病例并分析其病原分子N基因遗传进化关系。方法采集新疆阿勒泰地区疑似狂犬病羊脑组织,以狂犬病病毒特异性目的基因(N基因)RT-PCR扩增和序列测定进行病毒鉴定;以DNAStar-Lasergene.v7.1软件拼接测定序列,应用BLAST软件分析N基因的遗传进化关系。结果显示本研究鉴定的狂犬病毒阳性并获得了病毒全基因序列,建立了其N基因的遗传进化关系树,确定疑似的羊狂犬病病毒与狂犬病病毒基因Ⅰ型俄罗斯C群的遗传关系较近。结论应用RT-PCR方法对羊源狂犬病进行实验室诊断,获得该病原全基因序列,明确了新疆狂犬病病原和流行地域分布,为羊狂犬病毒分子流行病学研究奠定基础。To diagnose suspected sheep rabies case and analyze molecular epidemiological characteristics of the pathogen,brain tissues of sheep with clinical symptom from a farm in Xinjiang Uygur Autonomous Region,China were used.Rabies virus （RV） in brain tissue was confirmed by specific nucleoprotein gene （N） RT-PCR amplification.The full-length gene sequence of RV from sheep was sequenced and assembled by DNAStar Lasergene.V7.1 based on N gene sequence of rabies virus,which was analyzed with phylogenetic tree.Results showed that the sheep disease case was caused by RV.And the full-length sequence of RV from sheep in Xinjiang was obtained.It was suggested that the RV had a closer genetic relationship with the genotype I of Russia Group C by phylogenetic tree analysis.This study provided a basis for further study toward molecular epidemiology of sheep RV in China in the future.

关 键 词：羊 狂犬病病毒 N基因 进化树 

分 类 号：R373[医药卫生—病原生物学]