HMGA1通过Wnt/β-Catenin通路被诱导并维持胃癌细胞增殖  

HMGA1 is induced by Wnt /β-Catenin pathway and maintains cell proliferation in gastric cancer

作　　者：朱海涛[1] 郑志超[1] 张剑军[1] 刘晓平[1] 

机构地区：[1]辽宁省肿瘤医院胃外科,辽宁沈阳110042

出　　处：《中国微生态学杂志》2014年第5期511-513,525,共4页Chinese Journal of Microecology

基　　金：国家自然科学基金(81172047)

摘　　要：目的探讨HMGA1通过Wnt/β-Catenin通路在胃肿瘤形成中的作用。方法应用小干扰RNA介导的基因沉默、细胞增殖分析、PCR等技术完成实验。结果 HMGA1的特异敲除明显减少细胞生长。β-Catenin或下游c-myc的损失减少HMGA1表达,而Wnt3a处理增加HMGA1和c-myc的转录。结论这些数据表明,HMGA1参与胃癌细胞形成和增殖通过Wnt/β-Catenin通路。Objective To investigate the role of HMGA1 by Wnt/β-Catenin pathway in gastric tumor formation. Methods Adenovirus transfer, cell proliferation assay, and RT-PCR were used to perform the experiment. Resuits Specific knockdown of HMGA1 resulted in marked reduction of cell growth. The loss of β-catenin or its downstream c-myc decreased HMGA1 expression, whereas Wnt3a treatment increased HMGA1 and c-myc transcripts. Furthermore, Wnt/β-induced expression of HMGA1 was inhibited by c-myc knockdown, suggesting that HMGA1 is a downstream target of the Wnt/β-catenin pathway. Enhanced expression of HMGA1 coexisted with the nuclear accumulation of a-catenin in about 30% of gastric cancer tissues. Conclusion These data suggest that HMGA1 is involved in the proliferation and gastric tumor formation via the Wnt/β-catenin pathway.

关 键 词：HMGA1 WNT β-Catenin通路 胃肿瘤 

分 类 号：R735.2[医药卫生—肿瘤]