α-硫辛酸对帕金森病大鼠脑内多巴胺能神经元的保护作用及其机制探讨  

To explore mechanism of theα-lipoic acid neuroprotection effect in Parkinson's rats induced by rotenone

作　　者：王伟[1] 刘汉杰[2] 吴艳芬[1] 王永春[3] 

机构地区：[1]邯郸市第一医院,河北邯郸056001 [2]河北医科大学第二医院脑外科,河北石家庄050071 [3]邯郸市中医院,河北邯郸056001

出　　处：《中风与神经疾病杂志》2014年第5期419-421,共3页Journal of Apoplexy and Nervous Diseases

摘　　要：目的观察α-硫辛酸(LA)对鱼藤酮致帕金森(PD)大鼠模型黑质多巴胺能神经元的保护作用。方法健康成年雄性Wistar大鼠背部皮下注射鱼藤酮制备PD大鼠模型,药物治疗组同时给予大鼠腹腔注射LA。采用分光光度法检测大鼠脑内丙二醛(MDA)和还原型谷胱甘肽(GSH),Western blot检测大鼠中脑黑质及纹状体酪氨酸羟化酶(TH)的表达变化。结果与对照组相比,鱼藤酮组大鼠纹状体中MDA明显增高(P<0.01)而GSH含量明显降低(P<0.01),TH蛋白在黑质和纹状体与对照组比较明显降低(P<0.05);LA干预后与鱼藤酮组相比MDA明显降低及GSH明显增高(P<0.05),TH蛋白表达明显增加(P<0.05)。结论氧化应激在PD发病中起着非常重要的作用,LA能有效减轻PD大鼠脑内氧化应激损伤、保护脑内多巴胺能神经体系,同时改善PD样症状。Objective To investigate the protect effect ofα-lipoic acid（ LA） on the substantial nigra dopaminergic neuron in Parkinsons＇ rats induced by rotenone. Methods The healthy adult male Wistar rats were injected subcutaneously rotenone in back to prepare the PD rats model,the rats in drug therapy group were injected intraperitoneally LA at the same time. Spectrophotometry was used to detect oxidative stress parameters in rats（ MDA and GSH）,Westen blot was used to detect the changed expression of TH in the substantia nigra and striatum of rats. Results MDA was significantly increased（ P 0. 01）,and GSH was significantly decreased（ P 0. 01） in the rats striatum of rotenone group than that in control group. The expression of TH was significantly decreased in the substantia nigra and striatum compared with control group（ P 0. 05）. MDA were obviously decreased and GSH were increased significantly after the intervention of LA compared with rotenone group（ P 0. 05）,TH protein was increased significantly（ P 0. 05）. Conclusions Oxidative stress plays a very important role in the pathogenesis of PD,LA can effectively reduce the injury of dopaminergic neurons in rat brain,improve the symptoms of PD at the same time.

关 键 词：帕金森病 多巴胺 Α-硫辛酸 氧化应激 

分 类 号：R742.5[医药卫生—神经病学与精神病学]