慢性间歇性缺氧对幼年大鼠认知功能的影响及机制研究  

Influence of intermittent hypoxia on cognitive function on juvenile rats and associated mechanism

作　　者：罗斌[1] 任永生[1] 陈家强[1] 余广[2] 赵春玲[2] 

机构地区：[1]湖北医药学院基础医学院,十堰442000 [2]泸州医学院生理教研室

出　　处：《中华临床医师杂志（电子版）》2013年第10期115-117,共3页Chinese Journal of Clinicians(Electronic Edition)

摘　　要：目的观察慢性间歇性缺氧对幼年大鼠认知功能的影响并探讨其发生机制。方法 (20±2)日龄SD雄性大鼠40只,随机分为正常组和慢性间歇性缺氧组。将慢性间歇性缺氧组幼鼠置入自制缺氧舱内每天缺氧8 h,连续缺氧2周,建立模型后,利用Morris水迷宫检测认知功能的变化,记录海马CA1区神经元自发放电频率和幅度,利用HE染色和免疫组化法观察海马结构改变和GAP-43的表达情况。结果与正常组比较,慢性间歇性缺氧组体重较轻(P<0.05),在Morris水迷宫测试中逃避潜伏期明显延长(P<0.05),穿越平台次数明显减少(P<0.05);海马CA1区神经元自发放电频率、波幅减小(P<0.05),海马CA1区出现明显的损伤,GAP-43表达增高(P<0.05)。结论慢性间歇性缺氧可引起幼年大鼠认知功能损伤,而海马神经元突触可塑性的改变可能是认知功能损伤的机制。Objective To explore the effect of chronic intermittent hypoxia on cognitive function on juvenile rats and associated mechanism.Methods 40 SD male rats,（20 ±2）days old,were randomly divided into 2 groups：the control group and the chronic intermittent hypoxia （CIH） group.The CIH group were exposed to intermittent hypoxia in self-designed cabin for 8 h every day,and last 2 weeks.The cognitive function was assessed by the Morris Water Maze.Record the frequency and amplitude of neurons spontaneous discharge in hippocampus CA1 area.Using HE staining and immunohistochemical method to observe the change of hippocampus structure and the expression of GAP-43.Results Compared with the control group,the weight and cognitive impairment in the CIH group was statistically significant（P 〈 0.05）,the frequency and amplitude of neurons spontaneous discharge were decreased（P 〈 0.05）.Changes of Hippocampus structure were found.The expression of GAP-43 increased （P 〈 0.05）.Conclusion Chronic intermittent anaerobic can cause cognitive impairment on juvenile rats,and the high expression of GAP-43 may be the molecular mechanism.

关 键 词：睡眠呼吸暂停 阻塞性 认知 GAP-43蛋白 电生理学 

分 类 号：R363[医药卫生—病理学]