microRNA-181b影响U87胶质瘤细胞干细胞对替莫唑胺化疗耐受性的实验研究  

The study of the microRNA-181b affect U87 glioma stem cells on the chemotherapy tolerance to temozolomide

作　　者：李平[1,2] 赵兵[1,2] 吴德俊[1,2] 王少华[1,2] 李德坤[1,2] 江涛[1,2] 

机构地区：[1]安徽医科大学第二附属医院神经外科 [2]安徽医科大学脑血管病研究中心,合肥230601

出　　处：《中华临床医师杂志（电子版）》2013年第7期114-117,共4页Chinese Journal of Clinicians(Electronic Edition)

基　　金：安徽医科大学第二附属医院博士科研启动基金(2012BKJ006)

摘　　要：目的本研究旨在建立miR-181b慢病毒载体感染U87胶质瘤干细胞模型后,检测其对TMZ化疗耐受性的变化,并探讨miR-181b对于恶性脑胶质瘤(GBM)辅助化学治疗的意义。方法通过miR-181b慢病毒表达载体转染U87胶质瘤干细胞,提高miR-181b在U87胶质瘤干细胞中的表达。进一步通过RT-PCR、二次神经球形成实验、琼脂糖集落形成实验、MTT实验来分析miR-181b对于U87胶质瘤干细胞在TMZ化疗耐受性方面的作用。结果 miR-181b慢病毒表达载体转染U87胶质瘤干细胞后,U87胶质瘤干细胞中的miR-181b明显上调,而且U87胶质瘤干细胞的生长受到明显抑制;miR-181b可以加强TMZ抑制U87胶质瘤干细胞的生长的作用。结论 miR-181b可以加强TMZ抑制U87胶质瘤干细胞的生长,miR-181b可能对于提高U87胶质瘤干细胞对TMZ的敏感性,降低化疗耐受性起到一定作用。Objective To establish the miR-181b lentiviral vector infected the U87 glioma stem cells model,than detect the U87 glioma stem cells＇ chemotherapy tolerance to temozolomide （TMZ）,and to explore the significance of the miR-181 b for malignant glioma （gliomablastoma multiforme,GBM） in the adjuvant chemotherapy.Methods Using the miR-181b lentiviral vector transfected the U87 glioma stem cells and improve the level of miR-181b in the U87 glioma stem cells.RT-PCR,Secondary neurospheres formed assay,agarose colony formation assay and MTT assay were used to detect the role of the miR-181b affect the U87 glioma stem cells.in the TMZ chemotherapy tolerance.Results The level of miR-181b in the U87 glioma stem cells was significantly increased after miR-181b lentiviral vectors were transfected the U87 glioma stem cells,and the growth of U87 glioma stem cells were significantly inhibited.Furthermore,miR-181b can strengthen the role of TMZ in the growth inhibition of U87 glioma stem cells.Condusions We found that miR-181b can strengthen the role of TMZ in the growth inhibition of U87 glioma stem cells,miR-181 b may play a certain role of improving the sensitivity of U87 glioma stem cells to TMZ and reducing the chemotherapy tolerance.

关 键 词：微RNAS miR-181b 胶质瘤干细胞 替莫唑胺 化疗耐受 

分 类 号：R739.4[医药卫生—肿瘤]