基于^(131)I摄取功能的乳头状甲状腺癌肺转移患者血清差异miRNA的筛选及生物信息学分析  

Screening and bioinformatic analyses of serum microRNAs in papillary thyroid carcinoma with lung metastases based on iodine-131 uptake capacity

作　　者：薛艳丽[1] 邱忠领[2] 宋红俊[2] 罗全勇[2] 

机构地区：[1]苏州大学研究生部,江苏省215006 [2]上海交通大学附属第六人民医院核医学科

出　　处：《中华临床医师杂志（电子版）》2013年第1期58-61,共4页Chinese Journal of Clinicians(Electronic Edition)

基　　金：国家自然科学基金(81201115;81271611)

摘　　要：目的筛选与乳头状甲状腺癌(PTC)肺转移灶131I摄取功能相关的血清miRNAs。方法收集18例伴肺转移PTC患者血清标本,分为A、B两组,A组9例,为肺转移灶具有摄取131I功能者,B组9例,为肺转移灶无131I摄取功能者。采用miRNA芯片检测两组间的差异血清miRNAs,并经生物信息学分析筛选相关核心miRNA。结果通过miRNA的检测,发现A、B两组患者血清间具有显著差异的miRNAs13种,其中5种miRNAs在B组上调,8种miRNAs在B组下调。生物信息学分析发现血清miR-106a为核心miRNA,并参与调节193个靶基因,其中包括MAPK1、MAP3K8、MAP3K3、MAP3K12、MAP3K5、MAP3K14、MAP3K2、MAPK11等基因。结论 PTC肺转移灶具有131I摄取功能组患者血清与肺转移灶无131I摄取功能组患者血清miRNAs存在显著差异,在肺转移灶无131I摄取功能组患者血清中显著上调的miR-106a为核心miRNA,可能与肺转移灶丧失131I摄取功能有关。Objective To compare the differences in serum miRNAs between papillary thyroid carcinoma （PTC） patients with 131I-avid lung metastases and those with non-131I-avid lung metastases,and to screen a serum miRNA relevant to 131I uptake in lung metastases from PTC.Methods We collected serum samples from 9 PTC patients with 131I-avid lung metastases（Group A）and 9 PTC patients with non-131I-avid lung metastases（Group B）respectively,and both groups were matched for age and sex,without history of other tumors.The serum miRNAs were profiled using miRNA chip technology and the difference between the two groups was compared.Bioinformatics analysis was performed to screen the core miRNAs.Results A total of 13 serum miRNAs with statistically significant differences were detected between Group A and Group B.Among them,5 miRNAs displayed high expression,and 8 miRNAs showed low expression in Group B.Bioinformatics analysis showed that serum miR-106a was the core miRNA.The miR-106a was involved in regulating 193 target genes,including MAPK1,MAP3K8,MAP3K3,MAP3K12,MAP3K5,MAP3K14,MAP3K2,MAPK11 and et al.Conclusions The serum miRNAs were significantly different between PTC patients with 131I-avid lung metastases and those with non-131I-avid lung metastases.The miR-106a,which was significantly increased in patients with non-131I-avid lung metastases,was the core miRNA.Serum miR-106a might be associated with lung metastases losing the function of 131I uptake.

关 键 词：甲状腺肿瘤 乳头状瘤 肿瘤转移 微RNAS 

分 类 号：R736.1[医药卫生—肿瘤]