MiR-195对人脑胶质瘤细胞U251和SHG-44增殖的抑制作用  

MiR-195 inhibits the proliferation of human glioma cells

作　　者：慕伟[1] 苗旺[1] 刘晓东[1] 范益民[1] 王新星[1] 王宏勤[1] 李晋虎 

机构地区：[1]山西医科大学第一医院神经外科,太原030001

出　　处：《中华临床医师杂志（电子版）》2013年第12期103-105,共3页Chinese Journal of Clinicians(Electronic Edition)

基　　金：山西省基础研究计划(青年科技研究基金)项目(2012021035-4);山西省科技攻关项目(20110313011-3)

摘　　要：目的探讨miR-195过表达对体外培养的人脑胶质瘤细胞U251和SHG-44增殖的影响。方法通过脂质体将miR-195 mimics转染至U251和SHG-44细胞,同时设立空白对照组和阴性对照组,实时荧光定量PCR检测转染前后miR-195的表达水平;用CCK-8法评价细胞的增殖能力;流式细胞仪分析细胞周期。结果胶质瘤细胞转染miR-195 mimics后,发现U251细胞和SHG-44细胞中miR-195的表达分别为5.93±0.43和6.43±0.48,较空白对照组和阴性对照组均增高约6倍左右。与空白对照组和阴性对照组相比,转染miR-195 mimics后的胶质瘤细胞的增殖能力明显受到抑制,细胞周期阻滞于G0/G1期,S期细胞减少。结论过表达miR-195可以阻滞胶质瘤细胞周期进展,从而抑制其增殖,为胶质瘤的基因治疗提供新的策略。Objective To explore the effect of miR-195 on proliferation of U251 and SHG-44 glioblastoma cells.Methods miR-195 mimics were transfected into U251 and SHG-44 cells by Lipofectamine RNAiMAX .At the same time, the blank control group and negative control group were established .miR-195 expression level was identified by quantitative real-time polymerase chain reaction （ qRT-PCR ） .The ability of cell proliferation was detected by CCK8.Using flow cytometry to test and analyse cell cycle between the different groups .Results After transfection of miR-195 mimics, Real-time PCR showed that miR-195 expression level increased significantly . Compared with the blank control group and negative control group , after over-expression of miR-195 , glioma cells proliferation was obviously inhibited .By flow cytometry on cell cycle analysis , results showed that cell cycle was arrested in G0/G1 phase and S phase cells were decreased .Conclusion Over-expression of miR-195 could inhibit proliferation,block G1/S transition.miR-195 may play a potential role in glioma gene therapy .

关 键 词：神经胶质瘤 微RNA-195 细胞增殖 细胞周期 

分 类 号：R739.4[医药卫生—肿瘤]