水蛭提取液对体外培养LEC在IOL表面黏附的抑制作用  

Inhibition of extracting solution of Hirudo on the adherence to intraocular lens of bovine lens epithelial cells cultivated in vitro

作　　者：周春阳[1] 郑燕林[1] 周华祥[1] 

机构地区：[1]成都中医药大学附属医院眼科,四川成都610072

出　　处：《中医眼耳鼻喉杂志》2011年第2期87-89,共3页Journal of Chinese Ophthalmology and Otorhinolaryngology

摘　　要：目的 观察水蛭提取液对体外培养的牛晶状体上皮细胞（LEC）在人工晶状体（IOL）表面黏附生长的影响,探讨中药水蛭用于防治后发性白内障的可能性.方法 接种体外培养的LEC到PMMA材质的IOL表面,并加入高剂量和低剂量水蛭提取液.培养24 h后,观察IOL表面LEC的黏附生长抑制情况.结果 与空白组比较,水蛭提取液对LEC在IOL表面黏附生长有明显抑制作用（P《0.01）.结论 水蛭提取液能抑制体外培养的LEC在IOL表面黏附生长,实验为水蛭提取液用于防治后发性白内障提供了实验依据.Objective To examine the effects of extracting solution of Hirudo on the adherence to intraocular lens（IOL） of bovine lens epithelial cells（LEC） cultivated in vitro, for evaluating the possibility of Hirudo on the prevention of after cataract. Methods LEC was incubated with IOL in high and low dosage of extracting solution for 24h to investigate the inhibitive rate of cell adherence on the surface of PMMA IOL. Results There was significant inhibition to LEC on adherence on IOL of extracting solution of Hirudo （P^O. 01）. Conclusion Extracting solution of Hirudo can significantly inhibit adherence to IOL of LEC cultivated in vitro at the same time. This study gives some experiment foundation for extracting solution of Hirudo to prevent after cataract.

关 键 词：晶状体上皮细胞 水蛭 细胞培养 人工晶状体 后发性白内障 

分 类 号：R77[医药卫生—眼科]