CHFR基因在B细胞淋巴瘤细胞中的表达及意义(英文)  

The Expression and Significance of CHFR in B-cell Lymphoma Cells

作　　者：王红芳[1] 宋爱琴[1] 孙立荣[1] 叶俊丽[2] 邢晓明[3] 杨堃[4] 张敏[1] 

机构地区：[1]青岛大学附属医院血液儿科,山东青岛266003 [2]青岛大学医学院病理生理学教研室,山东青岛266021 [3]青岛大学附属医院病理科,山东青岛266003 [4]青岛大学附属医院中心实验室,山东青岛266003

出　　处：《现代生物医学进展》2014年第12期2242-2246,共5页Progress in Modern Biomedicine

基　　金：The Natural Science Foundation of China(31100824);The Natural Science Foundation of Shandong(Y2008c170);The Natural Science Foundation of Qingdao(2012-1-3-2-(5)-nsh)~~

摘　　要：目的:探讨CHFR基因在B细胞淋巴瘤Raji细胞中的表达,以及CHFR基因甲基化对Raji细胞增殖和凋亡中所产生的影响。方法:体外培养人B细胞淋巴瘤细胞株Raji细胞,用不同浓度的去甲基化试剂5-氮杂-2脱氧胞苷处理Raji细胞株,通过RT-PCR检测CHFR基因表达水平的变化,通过MS-PCR检测CHFR基因甲基化变化,CCK法及流式细胞术检测Raji细胞增殖及凋亡变化。结果:CHFR基因在Raji细胞中出现弱表达,经去甲基化试剂处理后CHFR基因表达水平增高,随药物浓度增加Raji细胞的抑制率及凋亡率增高。结论:5-氮杂-2脱氧胞苷可以恢复CHFR基因表达水平,抑制Raji细胞的增殖,促进凋亡。CHFR基因在细胞增殖起负向调控的作用。Objective：To investigate mRNA expression level of CHFR gene in lymphoma cells,the effect of CHFR gene mathylation and methylation inhibition on Raji cells proliferation and apoptosis.Methods：The human Burkitt＇s Raji lymphoma cells were cultivated in vitro.Raji cells were treated in different concentration of methylation inhibitor 5- Aza-2＇-deoxycytidine（5-Aza-dC）,then the expression level change of CHFR gene was detected by RT-PCR and methylation of CHFR gene was analyzed by MS-PCR.The cells proliferation of Raji cells treated with different concentrations of 5-Aza-dC were analyzed by CCK assay.The apoptosis of Raji cells was analyzed by flow cytometry.Results：CHFR gene had low-expression in Raji cells.5-Aza-dC can up-regulate the expression of CHFR mRNA by demethylation.The growth of Raji cells was inhibited and the apoptosis was promoted by 5-Aza-dC.Conclusion：The expression of CHFR can be recovered by the methylation inhibition.CHFR gene can inhibit growth in Raji cells,and it may play a negative role during the growth and proliferation.

关 键 词：CHFR基因 RAJI细胞 5-氮杂-2脱氧胞苷 甲基化 增殖 凋亡 

分 类 号：R733.4[医药卫生—肿瘤]