miRNA-125b在膀胱癌中的表达和意义  

Expression and Clinical Significance of miR-125b in Bladder Cancer

作　　者：任玉欣[1] 赵松[2] 付英梅[3] 刘云力[4] 罗娜[4] 赵柏[4] 

机构地区：[1]哈尔滨医科大学附属第一医院肿瘤一科,黑龙江哈尔滨150001 [2]哈尔滨医科大学附属第一医院普外三科,黑龙江哈尔滨150001 [3]哈尔滨医科大学微生物教研室,黑龙江哈尔滨150086 [4]哈尔滨医科大学附属第二医院泌尿外科,黑龙江哈尔滨150081

出　　处：《现代生物医学进展》2014年第12期2251-2254,共4页Progress in Modern Biomedicine

摘　　要：目的:探讨膀胱癌组织中miR-125b的表达在膀胱癌发生发展中的作用及其临床意义。方法:采用应用茎环RT-PCR的方法检测66例膀胱尿路上皮癌组织标本的miR-125b的表达,另有16例正常膀胱黏膜组织作对照,并结合临床病理资料进行统计学分析。结果:miR-125b在膀胱癌组织中的表达显著高于非肿瘤的正常膀胱黏膜组织(p<0.05),miR-125b的水平还与表达水平与膀胱癌的组织学分级、肿瘤转移、术后复发均明显相关性(P<0.05)。结论:miR-125b在膀胱癌组织中表达量升高,且与组织学分级、肿瘤转移、术后复发相关,可能作为膀胱癌诊断和预后指标。Objective：To investingate miR-125b expression in bladder cancer and to evaluate the potential correlations between miR-125b expression and clinicopathologic features of bladder cancer patients.Methods：Total RNA from 66 bladder cancer tissues and 16 normal bladder tissues were extracted and analyzed form miR-125b expression by stem-loop real-time RT-PCR.The correlation between miR-125b expression and clinicopathologic features was investigated.Results：miR-125b expression in bladder cancer tissues was significantly increased than those in those of normal bladder tissues（P〈0.05）.And expression level of miR-125b was related to histological grade,metastasis and recurrence（P〈0.05）.Conclusion：miR-125b has a higher expression level in bladder cancer.Expression level of miR-125b also has a strong association with histological grade,metastasis and recurrence in bladder cancer.And it has a potential application working as a diagnostic and prognostic indicator in bladder cancer.

关 键 词：膀胱癌 miR-125b 表达 意义 

分 类 号：Q95-3[生物学—动物学] R737.14[医药卫生—肿瘤]